Ollivier F J, Brooks D E, Kallberg M E, Sapp H L, Komáromy A M, Stevens G R, Dawson W W, Sherwood M B, Lambrou G N
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Vet Ophthalmol. 2004 Jan-Feb;7(1):23-7. doi: 10.1111/j.1463-5224.2004.00316.x.
To detect and categorize time-specific variations in daytime intraocular pressure (IOP) found in Rhesus monkeys with laser-induced ocular hypertension.
Ten male monkeys with argon laser-induced ocular hypertension in one eye were anesthetized with ketamine hydrochloride, and the IOP measured in both eyes at 7 a.m., 7.30 a.m., and then hourly until 1 p.m. with a Tonopen trade mark XL applanation tonometer. Intraocular pressure time profiles for both eyes in each animal were developed. The means +/- SD of the IOPs for both eyes were calculated for the whole 6-h study period, and the values compared statistically. The difference between the lasered eye mean IOP standard deviation and the normal eye mean IOP standard deviation for each animal during the 6-h follow-up was also calculated and compared.
Mean IOP (+/- SD) in the glaucoma and normal eyes for the 10 animals during the 6-h study was 32.6 +/- 2.5 and 14.9 +/- 2.5 mmHg, respectively. The IOP was significantly higher in the experimental eye than in the normal eye (P = 0.0008). The mean IOP in the lasered eye did not significantly change during the study period, whereas a slight but significant increase in IOP of the normal eye over the study period was recorded (P = 0.003). The variance in IOP in the hypertensive eyes was considerably greater than that in the untreated control eyes. From 7 a.m. to 1 p.m. the IOP declined in five eyes and increased in the other five eyes with laser-induced ocular hypertension.
The time-specific IOP variation pattern in the daytime in the laser treated eyes is significantly greater than the variation in the normotensive eyes. This shows that in order to detect statistical differences between IOP variations induced by an IOP-reducing drug, and the exaggerated spontaneous IOP variations present in the laser-induced hypertensive eye, sufficient animals should be included in any study. Understanding the time-specific IOP variation present in a group of monkeys with laser-induced ocular hypertension is essential prior to using the model for the evaluation of IOP-reducing drugs.
检测并分类激光诱导性高眼压恒河猴白天特定时间的眼压（IOP）变化情况。
对10只单眼经氩激光诱导性高眼压的雄性猴子用盐酸氯胺酮麻醉，于上午7点、7点30分用Tonopen商标XL压平眼压计测量双眼眼压，之后每小时测量一次直至下午1点。绘制每只动物双眼的眼压时间曲线。计算整个6小时研究期间双眼眼压的均值±标准差，并进行统计学比较。还计算并比较了每只动物在6小时随访期间激光治疗眼眼压均值标准差与正常眼眼压均值标准差的差异。
在6小时研究期间，10只动物青光眼眼和正常眼的平均眼压（±标准差）分别为32.6±2.5和14.9±2.5 mmHg。实验眼眼压显著高于正常眼（P = 0.0008）。研究期间激光治疗眼的平均眼压无显著变化，而正常眼眼压在研究期间有轻微但显著的升高（P = 0.003）。高眼压眼的眼压方差明显大于未治疗对照眼。上午7点至下午1点，5只激光诱导性高眼压眼的眼压下降，另外5只升高。
激光治疗眼白天特定时间的眼压变化模式显著大于正常眼压眼。这表明，为了检测降低眼压药物诱导的眼压变化与激光诱导性高眼压眼中存在的过度自发性眼压变化之间的统计学差异，任何研究都应纳入足够数量的动物。在使用该模型评估降低眼压药物之前，了解一组激光诱导性高眼压猴子中存在的特定时间眼压变化至关重要。
