铁螯合作用可调节紫外线A诱导的人重建表皮中的脂质过氧化和铁蛋白表达。
Iron chelation can modulate UVA-induced lipid peroxidation and ferritin expression in human reconstructed epidermis.
作者信息
Seité Sophie, Popovic Evelyne, Verdier Marie Paule, Roguet Roland, Portes Pascal, Cohen Catherine, Fourtanier Anny, Galey Jean Baptiste
机构信息
L'Oréal Recherche, Clichy, France.
出版信息
Photodermatol Photoimmunol Photomed. 2004 Feb;20(1):47-52. doi: 10.1111/j.1600-0781.2004.00064.x.
BACKGROUND/PURPOSE: As ferritin has been identified as an important factor in antioxidant defense in cultured human skin cells, we evaluated UVA-induced lipid hydroperoxides (LPO) production and ferritin expression in reconstructed human epidermis in vitro.
RESULTS
Ferritin is regularly present in the basal layer of unirradiated epidermis both in the human skin in vivo and in the reconstructed human epidermis in vitro. Following acute UVA exposure, ferritin expression increased in basal epidermal cells in both models. Quantitative analysis showed that, in reconstructed human epidermis, LPO and ferritin levels increased linearly with the UVA dose. An iron chelator, OR10141, inhibited these inductions.
CONCLUSION
These findings demonstrate that reconstructed human epidermis is a useful in vitro model to study UVA-induced oxidative stress and protection afforded by iron chelators, antioxidants or UVA absorbers.
背景/目的：由于铁蛋白已被确定为培养的人皮肤细胞抗氧化防御中的一个重要因素，我们在体外评估了紫外线A（UVA）诱导的脂质氢过氧化物（LPO）生成以及重组人表皮中铁蛋白的表达。
结果
在体内的人皮肤以及体外的重组人表皮中，铁蛋白均正常存在于未受照射表皮的基底层。急性UVA照射后，两种模型的基底表皮细胞中铁蛋白表达均增加。定量分析表明，在重组人表皮中，LPO和铁蛋白水平随UVA剂量呈线性增加。一种铁螯合剂OR10141可抑制这些诱导作用。
结论
这些发现表明，重组人表皮是研究UVA诱导的氧化应激以及铁螯合剂、抗氧化剂或UVA吸收剂所提供保护作用的一种有用的体外模型。
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