Rajlakshmi D, Banerjee S K, Sood S, Maulik S K
Department of Pharmacology, All India Institute of Medical Sciences, New Delhi - 110029, India.
J Pharm Pharmacol. 2003 Dec;55(12):1681-6. doi: 10.1211/0022357022296.
The in-vitro antioxidant activities of different concentrations of the water, alcoholic, petroleum ether and ethyl acetate extracts of the dried leaves of Clerodendron colebrookianum Walp, and in-vivo antioxidant activity of the water extract was studied in experimental rat models. The results obtained from in-vitro lipid peroxidation induced by FeSO4-ascorbate in rat liver homogenate showed a significant inhibition of lipid peroxidation by different extracts of C. colebrookianum leaf. Water extracts at concentrations (w/v) of 1:30, 1:50, 1:200 and 1:1000 showed the strongest inhibitory activity over the other organic extracts, suggesting maximum antioxidant effect. Chronic feeding of the water extract to Wistar albino rats (both sexes, 150-200 g) in 1 or 2 g kg-1/day doses for 14 days significantly increased the ferric reducing ability of plasma by 19% and 40% on the seventh day, and by 45% and 57% on the fourteenth day of treatment, respectively. Thiobarbituric acid reactive substances (TBARS), as a marker of lipid peroxidation, and some cellular antioxidants (superoxide dismutase, catalase and reduced glutathione) were estimated in heart, liver and kidney. There was a significant reduction in hepatic and renal TBARS with both the doses, without any change in myocardial TBARS. There was no change in the level of antioxidants in heart, liver and kidney, except for the hepatic superoxide dismutase. The findings of this study showed that the leaf extract of C. colebrookianum increased the antioxidant capacity of blood and had an inhibitory effect on the basal level of lipid peroxidation of liver and kidney. This lends scientific support to the therapeutic use of the plant leaves, as claimed by the tribal medicine of North-East India.
研究了不同浓度的臭牡丹（Clerodendron colebrookianum Walp）干燥叶片的水提取物、乙醇提取物、石油醚提取物和乙酸乙酯提取物的体外抗氧化活性，以及水提取物在实验大鼠模型中的体内抗氧化活性。由硫酸亚铁-抗坏血酸诱导大鼠肝匀浆体外脂质过氧化实验结果表明，臭牡丹叶的不同提取物对脂质过氧化有显著抑制作用。浓度（w/v）为1:30、1:50、1:200和1:1000的水提取物对脂质过氧化的抑制活性最强，表明其抗氧化效果最佳。以1或2 g kg⁻¹/天的剂量将水提取物长期喂饲给体重150 - 200 g的Wistar白化大鼠（雌雄皆有）14天，在治疗第7天时，血浆铁还原能力分别显著提高了19%和40%，在第14天时分别提高了45%和57%。测定了心脏、肝脏和肾脏中的硫代巴比妥酸反应物质（TBARS，作为脂质过氧化的标志物）以及一些细胞抗氧化剂（超氧化物歧化酶、过氧化氢酶和还原型谷胱甘肽）。两种剂量的提取物均使肝脏和肾脏中的TBARS显著降低，而心肌中的TBARS没有变化。除肝脏超氧化物歧化酶外，心脏、肝脏和肾脏中的抗氧化剂水平没有变化。本研究结果表明，臭牡丹叶提取物可提高血液的抗氧化能力，并对肝脏和肾脏脂质过氧化的基础水平有抑制作用。这为印度东北部部落医学所宣称的该植物叶的治疗用途提供了科学支持。
