Srivivasan Anusuya, Menon Venugopal P, Periaswamy Viswanathan, Rajasekaran K N
Department of Biochemistry, Annamalai University, Annamalai Nagar, India.
J Pharm Pharm Sci. 2003 Sep-Dec;6(3):327-33.
To evaluate the antioxidant defense by bis-o-hydroxycinnamoylmethane, analogue of the naturally occurring curcuminoid bis-demethoxycurcumin in streptozotocin induced diabetes in male Wistar rats and its possible protection of pancreatic beta-cell against gradual loss under diabetic condition.
Male wistar rats were divided into five groups. Group1 served as control rats. Group2 was control rats treated intragastrically with bis-o-hydroxycinnamoyl methane at a dose of 15 mg/kg body weight for 45 days. Group3, 4 and 5 rats were injected with 40 mg /kg body weight of streptozotocin to induce diabetes. Group4 rats were treated with the drug similar to group2 and group5 rats treated with the reference drug glibenclamide intragastrically for a similar period. After 45 days, the levels of plasma glucose, glycated hemoglobin, enzymic antioxidants (SOD, CAT) and non-enzymic antioxidants Vit C, Vit E was determined. Histopathological sections of the pancreas were examined.
The levels of plasma glucose and glycated hemoglobin which were elevated in group3 diabetic rats were reduced after treatment with the drug. The antioxidant levels showed an increase in the case of treated diabetic rats as compared to group3 diabetic rats. The islets were shrunken in group3 diabetic rats in comparison to normal rats. In the treated diabetic rats there was expansion of islets.
The experimental drug bis-o-hydroxycinnamoylmethane enhances the antioxidant defense against reactive oxygen species produced under hyperglycemic conditions and thus protects the pancreatic b -cell against loss and exhibits antidiabetic property.
评估双邻羟基肉桂酰甲烷（天然存在的姜黄素类双去甲氧基姜黄素的类似物）对链脲佐菌素诱导的雄性Wistar大鼠糖尿病的抗氧化防御作用，以及其在糖尿病状态下对胰腺β细胞逐渐丧失的可能保护作用。
雄性Wistar大鼠分为五组。第1组作为对照大鼠。第2组为对照大鼠，以15 mg/kg体重的剂量胃内给予双邻羟基肉桂酰甲烷，持续45天。第3、4和5组大鼠注射40 mg/kg体重的链脲佐菌素以诱导糖尿病。第4组大鼠以与第2组相似的药物进行治疗，第5组大鼠以参考药物格列本脲进行胃内给药，给药时间相似。45天后，测定血浆葡萄糖、糖化血红蛋白、酶促抗氧化剂（超氧化物歧化酶、过氧化氢酶）和非酶促抗氧化剂维生素C、维生素E的水平。检查胰腺的组织病理学切片。
第3组糖尿病大鼠升高的血浆葡萄糖和糖化血红蛋白水平在药物治疗后降低。与第3组糖尿病大鼠相比，治疗后的糖尿病大鼠抗氧化剂水平有所增加。与正常大鼠相比，第3组糖尿病大鼠的胰岛萎缩。在治疗后的糖尿病大鼠中，胰岛有扩张。
实验药物双邻羟基肉桂酰甲烷增强了对高血糖条件下产生的活性氧的抗氧化防御，从而保护胰腺β细胞免受损失，并表现出抗糖尿病特性。
