Tervahartiala P, Kivisaari L, Kivisaari R, Virtanen I
Department of Diagnostic Radiology, Helsinki University Central Hospital, Finland.
Invest Radiol. 1992 Dec;27(12):1064-8. doi: 10.1097/00004424-199212000-00019.
Contrast media-induced renal morphologic changes were studied in rats. Hemorrhagic pancreatitis was induced as a means of sensitizing the animals to the effects of contrast media.
The histologically verified hemorrhagic pancreatitis was induced in Wistar rats (n = 66) by injecting 6% sodium taurocholate into the pancreatic duct. After 2 hours, the animals received intravenously 1.0 or 3.0 g iodine/kg of high-osmolal osmolal diatrizoate, low-osmolal iopromide or iohexol, iso-osmolal iotrolan or 0.2 or 0.6 g/kg of high-osmolal magnetic resonance contrast agent, gadolinium-DTPA (Gd-DTPA). Control animals received physiologic saline. The kidneys were fixed by perfusion 2 hours later, and the morphologic changes were reviewed by two independent observers blinded to the injected agent.
The smaller dose of iohexol and the larger dose of all the contrast media induced a statistically significant (P < .001 or .01) cytoplasmic vacuolization in the proximal convoluted tubule (PCT) cells. The nonionic, low- and iso-osmolal contrast media caused as much or even significantly more vacuolization than diatrizoate.
Hemorrhagic pancreatitis potentiates the contrast media-induced renal morphologic changes, which depend on the type and dose of the injected contrast media.
研究了大鼠中造影剂诱导的肾脏形态学变化。诱导出血性胰腺炎作为使动物对造影剂作用敏感的一种手段。
通过向Wistar大鼠（n = 66）的胰管内注射6%牛磺胆酸钠诱导组织学证实的出血性胰腺炎。2小时后，动物静脉注射1.0或3.0 g碘/kg的高渗泛影葡胺、低渗碘普罗胺或碘海醇、等渗碘曲仑，或0.2或0.6 g/kg的高渗磁共振造影剂钆喷酸葡胺（Gd-DTPA）。对照动物接受生理盐水。2小时后通过灌注固定肾脏，由两名对注射药物不知情的独立观察者评估形态学变化。
较小剂量的碘海醇和所有造影剂的较大剂量在近曲小管（PCT）细胞中诱导出具有统计学意义（P <.001或.01）的细胞质空泡化。非离子型、低渗和等渗造影剂引起的空泡化与泛影葡胺相同甚至显著更多。
出血性胰腺炎增强了造影剂诱导的肾脏形态学变化，这取决于注射造影剂的类型和剂量。
