Tervahartiala P, Kivisaari L, Kivisaari R, Virtanen I
Department of Diagnostic Radiology, Helsinki University Central Hospital, Finland.
Acta Radiol. 1993 May;34(3):220-5.
Diabetes mellitus was induced in rats with streptozotocin and after 3 months the animals (n = 48) received an i.v. injection of 1 or 3 g I/kg in the form of high-osmolar diatrizoate, low-osmolar iopromide or iohexol, or of 0.6 g I/kg of high-osmolar Gd-DTPA. The controls were given an i.v. injection of physiologic saline. After 2 hours the kidneys were fixed by perfusion and the renal morphologic changes were semiquantitatively analyzed by two independent observers unaware of the agent administered. The contrast media (CM) induced pronounced cytoplasmic vacuolization in the proximal convoluted tubular cells. Such a lysosomal alteration may indicate CM uptake into the cell, and the ultrastructural evaluation revealed intracellular injuries related to the process. The alterations were most marked following administration of iohexol, but diatrizoate also induced a statistically highly significant vacuolization (p < 0.001). The lysosomal alterations following iopromide administration were not as striking, and Gd-DTPA induced only minor changes.
用链脲佐菌素诱导大鼠患糖尿病，3个月后，48只动物静脉注射1或3 g碘/千克，分别采用高渗泛影酸盐、低渗碘普罗胺或碘海醇的形式，或0.6 g碘/千克的高渗钆喷酸葡胺。对照组静脉注射生理盐水。2小时后通过灌注固定肾脏，由两名不知道所给予药物的独立观察者对肾脏形态学变化进行半定量分析。造影剂（CM）在近端曲管细胞中引起明显的细胞质空泡化。这种溶酶体改变可能表明CM被细胞摄取，超微结构评估显示与该过程相关的细胞内损伤。在给予碘海醇后改变最为明显，但泛影酸盐也引起了统计学上高度显著的空泡化（p < 0.001）。给予碘普罗胺后的溶酶体改变不那么显著，而钆喷酸葡胺仅引起轻微变化。
