Sueki Hirohiko, Kligman Albert M
Department of Dermatology Showa University School of Medicine, Tokyo, Japan.
J Dermatol. 2003 Dec;30(12):859-70. doi: 10.1111/j.1346-8138.2003.tb00340.x.
To evaluate the toxicity of irritant chemicals on animal skin, investigators have frequently had to apply high concentrations, owing to the fact that its susceptibility is less than that of human skin. High concentrations are so damaging to tissue that specific effects are obscured on the various layers. The aim of the present study was to elucidate the effects of a variety of irritating chemicals on the skin of hairless guinea pigs. Graded concentrations of these irritating substances were applied to the back for varying periods. Histologic changes were analyzed by light and electron microscopy. The structural alterations varied greatly among the chemicals, reflecting quite different mechanisms of action. Hairless guinea pigs are quite susceptible to chemical injury, especially to their hair follicles and dermal components. The hairless guinea pig appears to be an advantageous model to assess the acute and chronic effects of chemical irritants.
为评估刺激性化学物质对动物皮肤的毒性，研究人员常常不得不使用高浓度物质，因为动物皮肤的敏感性低于人类皮肤。高浓度物质对组织的损害极大，以至于各层的特定效应被掩盖。本研究的目的是阐明多种刺激性化学物质对无毛豚鼠皮肤的影响。将这些刺激性物质的不同浓度梯度涂抹于豚鼠背部不同时长。通过光学显微镜和电子显微镜分析组织学变化。不同化学物质之间的结构改变差异很大，这反映出其作用机制截然不同。无毛豚鼠对化学损伤相当敏感，尤其是对其毛囊和真皮成分。无毛豚鼠似乎是评估化学刺激物急性和慢性影响的有利模型。
