Park Eun-Kyung, Choo Min-Kyung, Han Myung Joo, Kim Dong-Hyun
College of Pharmacy, Kyung Hee University, Seoul, Korea.
Int Arch Allergy Immunol. 2004 Feb;133(2):113-20. doi: 10.1159/000076383. Epub 2004 Jan 21.
Ginseng (the root of Panax ginseng C.A. Meyer, Araliaceae) has been reported to possess various biological activities, including anti-inflammatory and antitumor actions. In this study, we investigated the antiallergic activity of ginsenosides isolated from ginseng.
We isolated ginsenosides by silica gel column chromatography and examined their in vitro and in vivo antiallergic effect on rat peritoneal mast cells and on IgE-induced passive cutaneous anaphylaxis (PCA) in mice. The in vitro anti-inflammatory activity of ginsenoside Rh1 (Rh1) in RAW264.7 cells was investigated.
Rh1 potently inhibited histamine release from rat peritoneal mast cells and the IgE-mediated PCA reaction in mice. The inhibitory activity of Rh1 (87% inhibition at 25 mg/kg) on the PCA reaction was found to be more potent than that of disodium cromoglycate (31% inhibition at 25 mg/kg); Rh1 was also found to have a membrane-stabilizing action as revealed by differential scanning calorimetry. It also inhibited inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression in RAW 264.7 cells, and the activation of the transcription factor, NF-kappaB, in nuclear fractions.
The antiallergic action of Rh1 may originate from its cell membrane-stabilizing and anti-inflammatory activities, and can improve the inflammation caused by allergies.
人参（五加科人参的根）据报道具有多种生物活性，包括抗炎和抗肿瘤作用。在本研究中，我们研究了从人参中分离出的人参皂苷的抗过敏活性。
我们通过硅胶柱色谱法分离人参皂苷，并检测它们对大鼠腹腔肥大细胞以及对小鼠IgE诱导的被动皮肤过敏反应（PCA）的体外和体内抗过敏作用。研究了人参皂苷Rh1（Rh1）在RAW264.7细胞中的体外抗炎活性。
Rh1能有效抑制大鼠腹腔肥大细胞释放组胺以及小鼠中IgE介导的PCA反应。发现Rh1对PCA反应的抑制活性（25mg/kg时87%的抑制率）比色甘酸钠（25mg/kg时31%的抑制率）更强；差示扫描量热法显示Rh1还具有膜稳定作用。它还抑制RAW 264.7细胞中诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）蛋白的表达，以及核组分中转录因子NF-κB的激活。
Rh1的抗过敏作用可能源于其细胞膜稳定和抗炎活性，并且可以改善由过敏引起的炎症。
