Hfq的一种新的伴侣作用：与信使核糖核酸结合决定小核糖核酸调节因子的作用途径
Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator.
作者信息
Geissmann Thomas A, Touati Danièle
机构信息
Institut Jacques Monod, CNRS-Universités Paris 6 et Paris 7, Paris, France.
出版信息
EMBO J. 2004 Jan 28;23(2):396-405. doi: 10.1038/sj.emboj.7600058. Epub 2004 Jan 22.
Abstract
The Sm-like protein Hfq is involved in post-transcriptional regulation by small, noncoding RNAs in Escherichia coli that act by base pairing. Hfq stabilises the small RNAs and mediates their interaction with the target mRNA by an as yet unknown mechanism. We show here a novel chaperoning use of Hfq in the regulation by small RNAs. We analysed in vitro and in vivo the role of Hfq in the interaction between the small RNA RyhB and its sodB (iron superoxide dismutase) mRNA target. Hfq bound strongly to sodB mRNA and altered the structure of the mRNA, partially opening a loop. This gives access to a sequence complementary to RyhB and encompassing the translation initiation codon. RyhB binding blocked the translation initiation codon of sodB and triggered the degradation of both RyhB and sodB mRNA. Thus, Hfq is a critical chaperone in vivo and in vitro, changing the folding of the target mRNA to make it subject to the small RNA regulator.
摘要
类Sm蛋白Hfq参与大肠杆菌中由小的非编码RNA通过碱基配对进行的转录后调控。Hfq可稳定小RNA，并通过一种尚不清楚的机制介导它们与靶标mRNA的相互作用。我们在此展示了Hfq在小RNA调控中的一种新的伴侣功能。我们在体外和体内分析了Hfq在小RNA RyhB与其sodB（铁超氧化物歧化酶）mRNA靶标之间相互作用中的作用。Hfq与sodB mRNA紧密结合并改变了mRNA的结构，部分打开了一个环。这使得与RyhB互补且包含翻译起始密码子的序列得以暴露。RyhB的结合阻断了sodB的翻译起始密码子，并引发了RyhB和sodB mRNA的降解。因此，Hfq在体内和体外都是一种关键的伴侣蛋白，它改变靶标mRNA的折叠，使其受到小RNA调控。
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