Department of Biology, Shantou University, Shantou, 515063, Guangdong, China.
Dongguan Nancheng Business District North School, Dongguan, 523000, China.
BMC Microbiol. 2024 Sep 7;24(1):330. doi: 10.1186/s12866-024-03486-z.
The RNA binding protein is crucial for gene regulation at the post transcription level. In this study, functions of the DUF1127-containing protein and ProQ, which are RNA-binding proteins, were revealed in Vibrio alginolyticus. DUF1127 deletion increased the ability of biofilm formation, whereas ProQ deletion reduced the amount of biofilm. Moreover, extracellular proteinase secretion was significantly reduced in the DUF1127 deletion strain. ProQ, not DUF1127-containing protein, can help the cell to defense oxidative stress. Deletion of DUF1127 resulted in a higher ROS level in the cell, however, ProQ deletion showed no difference. RNA-seq unveiled the expression of genes involved in extracellular protease secretion were significantly downregulated and biofilm synthesis-related genes, such as rbsB and alsS, were differentially expressed in the DUF1127 deletion strain. ProQ affected the expression of genes involved in biofilm synthesis (flgC and flgE), virulence (betB and hutG), and oxidative stress. Moreover, the DUF1127-containing and ProQ affected the mRNA levels of various regulators, such as LysR and BetI. Overall, our study revealed that the DUF1127-containing protein and ProQ have crucial functions on biofilm formation in V. alginolyticus.
RNA 结合蛋白在转录后水平的基因调控中至关重要。在这项研究中，揭示了含有 DUF1127 的蛋白和 ProQ（一种 RNA 结合蛋白）在解藻弧菌中的功能。DUF1127 缺失增加了生物膜形成的能力，而 ProQ 缺失则减少了生物膜的量。此外，DUF1127 缺失菌株的胞外蛋白酶分泌显著减少。ProQ 而不是含有 DUF1127 的蛋白可以帮助细胞抵御氧化应激。DUF1127 的缺失导致细胞内 ROS 水平升高，而 ProQ 的缺失则没有差异。RNA-seq 揭示了参与细胞外蛋白酶分泌的基因的表达显著下调，并且生物膜合成相关基因，如 rbsB 和 alsS，在 DUF1127 缺失菌株中差异表达。ProQ 影响生物膜合成（flgC 和 flgE）、毒力（betB 和 hutG）和氧化应激相关基因的表达。此外，含有 DUF1127 的蛋白和 ProQ 影响各种调节剂（如 LysR 和 BetI）的 mRNA 水平。总的来说，我们的研究表明，含有 DUF1127 的蛋白和 ProQ 在解藻弧菌的生物膜形成中具有重要功能。
