Sudhoff H, Liebehenz Y, Aschenbrenner J, Euteneuer S, Ebmeyer J, Bernal-Sprekelsen M, Stark T, Dazert S
Hals-Nasen-Ohrenklinik der Ruhr-Universität Bochum, St. Elisabeth Hospital, Bochum, Germany.
Laryngorhinootologie. 2004 Jan;83(1):14-9. doi: 10.1055/s-2004-814236.
The pathology associated to cholesteatoma is predominantly a consequence of osteoclast-mediated bone resorption within the middle ear. To assess its pathogenesis a murine model for dermal-implant induced osteolysis was evaluated for the expression of osteoclast stimulating and differentiating factors.
Mouse calvaria were analysed for the expression of osteoprotegerin ligand (OPGL), osteoprotegerin (OPG) and macrophage-colony stimulating factor (M-CSF) using immunohistochemistry. The detection of osteoclast cell lineage was acquired by immunohistochemistry using markers CD 4, CD 11a, CD 11b, CD 14, CD 51, CD 68 and TRAP.
An increased expression of the investigated cytokines M-CSF, OPG and OPGL was demonstrated by immunohistochemistry. The presence of osteoclast precursor cells and mature resorbing osteoclasts was confirmed in time-dependent manner triggered by dermal implantation.
This study reveals the basic events in osteoclast biology in localized inflammatory bone resorption and provides new insights into the comprehension of cholesteatoma-induced bone resorption.
