Sakata M, Masuko-Hongo K, Nakamura H, Onuma H, Tsuruha J I, Aoki H, Nishioka K, Kato T
Department of Bioregulation, Institute of Medical Science, St. Marianna University School of Medicine, Kanagawa, Japan.
Clin Exp Rheumatol. 2003 Nov-Dec;21(6):704-10.
To clarify the presence of specific T cell immune response to autologous chondrocytes in patients with osteoarthritis (OA).
Peripheral blood mononuclear cells obtained from OA or post-traumatic patients were co-cultured with irradiated autologous chondrocytes, and their proliferative response was assessed using 3H-thymidine incorporation. Expression of HLA-class II molecules was also assessed on chondrocytes by immunohistochemistry or flow cytometry.
T cell responses to autologous chondrocytes in OA yielded a significantly greater mean stimulation index (6.35 +/- 1.63) compared to controls (1.21 +/- 0.09, p < 0.01). This response was partially blocked by antibodies against HLA class I, class II, CD4 or CD8. Increased expression of HLA-DP, -DQ, and -DR was observed.
This study showed the autologous T cell-stimulating property of OA chondrocytes in vitro. The elucidation of the autoimmune responses may contribute to the understanding of immune-mediated mechanisms in OA.
阐明骨关节炎（OA）患者中针对自体软骨细胞的特异性T细胞免疫反应的存在情况。
从OA患者或创伤后患者获取外周血单个核细胞，将其与经辐照的自体软骨细胞共培养，使用³H-胸腺嘧啶核苷掺入法评估其增殖反应。还通过免疫组织化学或流式细胞术评估软骨细胞上HLA-II类分子的表达。
与对照组（1.21±0.09，p<0.01）相比，OA患者中针对自体软骨细胞的T细胞反应产生的平均刺激指数显著更高（6.35±1.63）。该反应被抗HLA-I类、II类、CD4或CD8抗体部分阻断。观察到HLA-DP、-DQ和-DR的表达增加。
本研究显示了OA软骨细胞在体外具有自体T细胞刺激特性。对自身免疫反应的阐明可能有助于理解OA中的免疫介导机制。
