Nakamura Hiroshi, Tanaka Michiaki, Masuko-Hongo Kayo, Yudoh Kazuo, Kato Tomohiro, Beppu Moroe, Nishioka Kusuki
Institute of Medical Science, St. Marianna University, Sugao, 216-8512 Kawasaki, Japan.
Rheumatol Int. 2006 Sep;26(11):984-90. doi: 10.1007/s00296-006-0116-5. Epub 2006 Feb 9.
It has been reported that T cells and chondrocytes interact through cell surface molecules such as MHC, CD4 or CD8 in osteoarthritis (OA) and T cells are activated. The objective of this study is to investigate the responses of chondrocyte-T cell interaction in terms of metalloprotease (MMP) and chemokine production. Articular cartilage and autologous blood were obtained from patients with OA and fracture who under went prosthetic surgery. Synovial fluid (SF) was collected from OA patients. Isolated chondrocytes were co-cultured with autologous T cells. SF cells were analyzed by immunostaining or Alcian blue staining. The production of MMP-1, MMP-3, MMP-13, and regulated on activation, normal T expressed and secreted (RANTES) was enhanced by direct co-culture compared to indirect co-culture using Transwell. Production ratio of RANTES in OA was significantly higher than non-arthritic samples. CD3 positive mononuclear cells and chondrocyte-like cells were found in SF. Chondrocyte-T cell contact was more adhesive in OA samples. These results showed the production of MMPs and RANTES was enhanced by the interaction and that chondrocyte-T cell contact was possible in vivo.
据报道，在骨关节炎（OA）中，T细胞与软骨细胞通过MHC、CD4或CD8等细胞表面分子相互作用，T细胞被激活。本研究的目的是从金属蛋白酶（MMP）和趋化因子产生方面研究软骨细胞 - T细胞相互作用的反应。从接受假体手术的OA患者和骨折患者身上获取关节软骨和自体血液。从OA患者身上收集滑液（SF）。将分离的软骨细胞与自体T细胞共培养。通过免疫染色或阿尔辛蓝染色分析SF细胞。与使用Transwell的间接共培养相比，直接共培养可增强MMP - 1、MMP - 3、MMP - 13以及激活调节正常T细胞表达和分泌因子（RANTES）的产生。OA中RANTES的产生比例显著高于非关节炎样本。在SF中发现了CD3阳性单核细胞和软骨样细胞。在OA样本中软骨细胞 - T细胞接触更具黏附性。这些结果表明，相互作用增强了MMPs和RANTES的产生，并且在体内软骨细胞 - T细胞接触是可能的。
