Hyllner S J, Haux C
Department of Zoophysiology, University of Göteborg, Sweden.
J Endocrinol. 1992 Nov;135(2):303-9. doi: 10.1677/joe.0.1350303.
The major vitelline envelope proteins were detected in the plasma of female rainbow trout maturing under natural conditions by using the Western blot technique. Females were sampled every month from July until ovulation in January. The amount of vitelline envelope proteins in plasma increased markedly as the gonads increased in size from 0.4 to about 15% of the total body weight. The plasma level of oestradiol-17 beta largely followed the alterations in the amount of vitelline envelope proteins, indicating the endocrine control of vitelline envelope protein synthesis. In addition, plasma vitellogenin changed in a manner that resembled the changes in the amount of plasma vitelline envelope proteins. The appearance and growth of the vitelline envelope during oocyte development was demonstrated using immunohistochemical methods. The vitelline envelopes from oocytes at different stages of development were immunoreactive with the antibodies directed against the major vitelline envelope proteins. No immunoreactivity could be observed in the ooplasm or in the surrounding follicular cells, which indicated that the major vitelline envelope proteins were of extraovarian origin. The present study further supports the hypothesis that the major protein constituents of the vitelline envelope in teleosts are under the endocrine control of oestradiol-17 beta and that the site of synthesis is outside the ovary.
利用蛋白质免疫印迹技术，在自然条件下成熟的雌性虹鳟血浆中检测到了主要的卵黄膜蛋白。从7月到1月排卵期间，每月对雌性虹鳟进行采样。随着性腺大小从占体重的0.4%增加到约15%，血浆中卵黄膜蛋白的含量显著增加。血浆中17β-雌二醇的水平在很大程度上随卵黄膜蛋白含量的变化而变化，表明卵黄膜蛋白合成受内分泌控制。此外，血浆卵黄蛋白原的变化方式与血浆卵黄膜蛋白含量的变化相似。利用免疫组织化学方法证明了卵母细胞发育过程中卵黄膜的出现和生长。不同发育阶段卵母细胞的卵黄膜与针对主要卵黄膜蛋白的抗体发生免疫反应。在卵质或周围卵泡细胞中未观察到免疫反应性，这表明主要的卵黄膜蛋白来自卵巢外。本研究进一步支持了这样的假说，即硬骨鱼类卵黄膜的主要蛋白质成分受17β-雌二醇的内分泌控制，且合成部位在卵巢外。
