Hyllner S J, Oppen-Berntsen D O, Helvik J V, Walther B T, Haux C
Department of Zoophysiology, University of Göteborg, Sweden.
J Endocrinol. 1991 Nov;131(2):229-36. doi: 10.1677/joe.0.1310229.
During growth of the ovarian follicle, the teleost oocyte becomes surrounded by an acellular coat, the vitelline envelope. The nature, origin and number of the vitelline envelope proteins in fish appear to vary with species. In this work, polyclonal antibodies directed against vitelline envelope proteins from rainbow trout, brown trout and turbot were used to show that oestradiol-17 beta induces the major vitelline envelope proteins in juveniles, both males and females, from different species. The fact that males can synthesize vitelline envelope constituents shows that the origin of these proteins is not confined to the ovary. The vitelline envelope of rainbow trout eggs consists of three major proteins, designated alpha (60 kDa), beta (55 kDa) and gamma (50 kDa). The amino acid composition of each of the three proteins indicated that the three proteins are alike and the suggestion that these proteins represent a separate class of structural proteins is sustained.
在卵巢卵泡生长过程中，硬骨鱼的卵母细胞被一层无细胞的包膜——卵黄膜所包围。鱼类卵黄膜蛋白的性质、来源和数量似乎因物种而异。在这项研究中，针对虹鳟、褐鳟和大菱鲆卵黄膜蛋白的多克隆抗体被用于证明，17β-雌二醇可诱导不同物种的幼年雄性和雌性产生主要的卵黄膜蛋白。雄性能够合成卵黄膜成分这一事实表明，这些蛋白质的来源并不局限于卵巢。虹鳟鱼卵的卵黄膜由三种主要蛋白质组成，分别命名为α（60 kDa）、β（55 kDa）和γ（50 kDa）。这三种蛋白质各自的氨基酸组成表明它们彼此相似，且这些蛋白质代表一类独立的结构蛋白这一观点得到了支持。
