Lankinen K S, Rintamäki S, Syrjänen R, Kilpi T, Ruutu P, Leinonen M
Department of Microbiology, KTL, National Public Health Institute, P.O. Box 310, FIN-90101, Oulu, Finland.
J Microbiol Methods. 2004 Feb;56(2):193-9. doi: 10.1016/j.mimet.2003.10.021.
We developed a new competitive EIA method for the demonstration of pneumococcal capsular polysaccharides from respiratory samples. The pediatric types 4, 6B, 9V, 14, 18C, 19F and 23F were selected for this study, because these capsular polysaccharides were included in the first heptavalent pneumococcal conjugate vaccines, which were used in the Finnish Otitis Media Vaccine Trial. Sensitivity of the EIA tests for purified polysaccharide antigens varied between 5 and 100 ng/ml, depending on the type. The assays performed well in 100 nasopharyngeal samples (NPS) samples processed through an enrichment culture, with an almost 100% sensitivity compared with routine culture. The method appeared type-specific, except that EIA for 6B capsule also detected 6A. The method is applicable for type-specific identification of pneumococcus in carriage studies.
我们开发了一种新的竞争性酶免疫测定（EIA）方法，用于检测呼吸道样本中的肺炎球菌荚膜多糖。本研究选择了儿童型4、6B、9V、14、18C、19F和23F，因为这些荚膜多糖包含在芬兰中耳炎疫苗试验中使用的首批七价肺炎球菌结合疫苗中。EIA检测纯化多糖抗原的灵敏度在5至100 ng/ml之间，具体取决于类型。该检测方法在通过富集培养处理的100份鼻咽样本（NPS）中表现良好，与常规培养相比，灵敏度几乎达到100%。该方法具有型特异性，不过6B荚膜的EIA也能检测到6A。该方法适用于携带研究中肺炎球菌的型特异性鉴定。
