Arakawa Keita, Hanazaki Mitsuru, Yoshida Shizuo
Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan.
Biosci Biotechnol Biochem. 2004 Jan;68(1):175-82. doi: 10.1271/bbb.68.175.
When plasma membranes were prepared from tubers of Helianthus tuberosus L. (Jerusalem artichoke) frozen at a sublethal temperature (-10 degrees C), the levels of some plasma membrane proteins, named frost-susceptible proteins (FSPs), decreased [Uemura, M., et al., Plant Physiol., 80, 187-195 (1986)]. The aim of this study was to characterize the response of FSP120, which is named FSP-3 in a previous report, to freezing treatment by immunoblotting. Levels of FSP120 in the plasma membranes of tubers decreased after sublethal freezing, whereas no degraded products were detected in the microsomes or the soluble fraction. The amount of FSP120 in the crude extract of frozen tubers remained at a comparable level to that of the unfrozen tubers. These results suggest that FSP120 might be released from plasma membranes during freezing treatment of the tubers of Jerusalem artichoke.
当从在亚致死温度（-10℃）下冷冻的菊芋块茎制备质膜时，一些名为霜冻敏感蛋白（FSPs）的质膜蛋白水平下降[上村，M.等人，植物生理学，80，187 - 195（1986）]。本研究的目的是通过免疫印迹法表征FSP120（在先前报告中称为FSP - 3）对冷冻处理的反应。亚致死冷冻后，块茎质膜中FSP120的水平下降，而在微粒体或可溶性部分中未检测到降解产物。冷冻块茎粗提物中FSP120的量与未冷冻块茎的量保持在相当水平。这些结果表明，在菊芋块茎冷冻处理过程中，FSP120可能从质膜中释放出来。
