Sugihara Satoru, Kondo Mariko, Chihara Yûko, Yuji Mami, Hattori Hiroyuki, Yoshida Munehiro
Laboratory of Food and Nutritional Sciences, Department of Biotechnology, Faculty of Engineering, Kansai University, Osaka, Japan.
Biosci Biotechnol Biochem. 2004 Jan;68(1):193-9. doi: 10.1271/bbb.68.193.
Sprouts of several plants (10 families and 28 species) were cultivated in a high selenium environment, and the chemical species of selenium in these selenium-enriched sprouts were identified by using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICPMS). Cultivation of sprouts of kaiware daikon (type of radish) with 5.0 microg/ml or 10.0 microg/ml of selenium as selenite inhibited the growth. However, no abnormalities in the shape or color were apparent even in the sprouts exposed to 10.0 microg/ml of selenium. The selenium concentration in the sprouts of most plants examined was higher than that from environmental exposure. Among the types of selenium that were accumulated, a large part (69-98%) was extractable in 0.2 M HCl. Chemical analysis of selenium in the HCl extract showed that the main selenium species in all the sprouts examined was Se-methylselenocysteine. In addition to Se-methylselenocysteine, selenomethionine, non-metabolized selenite, gamma-glutamyl-Se-methylselenocysteine and an unknown selenium compound were also detected in several high-selenium sprouts. Since higher anticarcinogenic activities of these monomethylated selenoamino acids have been observed, it is anticipated that such selenium-enriched sprouts will be used as a foodstuff for cancer prevention.
在高硒环境中培育了几种植物（10个科28个物种）的芽苗，并使用高效液相色谱 - 电感耦合等离子体质谱法（HPLC - ICPMS）鉴定了这些富硒芽苗中硒的化学形态。用5.0微克/毫升或10.0微克/毫升的亚硒酸盐作为硒源培育水萝卜（一种萝卜）芽苗时会抑制其生长。然而，即使是暴露于10.0微克/毫升硒的芽苗，其形状或颜色也没有明显异常。大多数被测植物芽苗中的硒浓度高于环境暴露情况下的浓度。在积累的硒类型中，很大一部分（69 - 98%）可在0.2 M盐酸中提取。对盐酸提取物中硒的化学分析表明，所有被测芽苗中的主要硒形态是甲基硒代半胱氨酸。除了甲基硒代半胱氨酸外，在几种高硒芽苗中还检测到了硒代蛋氨酸、未代谢的亚硒酸盐、γ - 谷氨酰 - 甲基硒代半胱氨酸和一种未知的硒化合物。由于已观察到这些单甲基化硒代氨基酸具有更高的抗癌活性，预计这种富硒芽苗将被用作预防癌症的食品。
