Nakahara Satoshi, Kusano Miyako, Fujioka Shozo, Shimada Atsumi, Kimura Yasuo
Department of Biological and Environmental Chemistry, Faculty of Agriculture, Tottori University, Tottori-shi, Japan.
Biosci Biotechnol Biochem. 2004 Jan;68(1):257-9. doi: 10.1271/bbb.68.257.
New acetylenic nematicidal compound, penipratynolene (1), methy (2'R)-4-(2'-hydroxy-3'-butynoxy)benzoate, together with two known compounds, 6-methoxycarbonylpicolinic acid (2) and 2,6-pyridinedicarboxylic acid (3), were isolated from the culture filtrate of Penicillium bilaiae Chalabuda. The structures of 1-3 were established by spectroscopic methods. The absolute configuration of 1 was confirmed by using a modified version of Mosher's method. Compounds 1-3 showed nematicidal activity of 77%, 52%, and 98%, respectively, by a bioassay at 300 mg/l with the root-lesion nematode Pratylenchus penetrans.
新型炔类杀线虫化合物青霉炔醇（1），即（2'R）-4-（2'-羟基-3'-丁炔氧基）苯甲酸甲酯，与两种已知化合物6-甲氧基羰基吡啶甲酸（2）和2,6-吡啶二甲酸（3），是从双孢青霉（Penicillium bilaiae Chalabuda）的培养滤液中分离得到的。通过光谱方法确定了1-3的结构。采用改良的莫舍尔方法确定了1的绝对构型。通过对根腐线虫穿刺短体线虫（Pratylenchus penetrans）进行300 mg/l的生物测定，化合物1-3的杀线虫活性分别为77%、52%和98%。
