Sitki-Green Diane L, Edwards Rachel Hood, Covington Mary M, Raab-Traub Nancy
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
J Infect Dis. 2004 Feb 1;189(3):483-92. doi: 10.1086/380800. Epub 2004 Jan 13.
Infectious mononucleosis is the clinical manifestation of primary infection with Epstein-Barr virus (EBV). We monitored primary infection during convalescence and during the establishment of persistent infection. The profiles of EBV strains in the oral cavity and in peripheral blood were determined by use of a heteroduplex tracking assay specific for the EBV gene encoding latent membrane protein 1. Multiple EBV strains were detected in most patients and persisted in and were possibly transmitted among 3 distinct compartments of infection, including the oral cavity, peripheral blood lymphocytes, and the cell-free fraction of the blood plasma. We also tracked transmission of multiple strains from an asymptomatic carrier to a patient diagnosed with primary EBV infection. These data reveal that primary EBV infection is complex, with transmission of multiple strains and clear differences in relative abundance of strains in distinct compartments.
传染性单核细胞增多症是由爱泼斯坦-巴尔病毒（EBV）原发感染引起的临床表现。我们在恢复期以及持续性感染确立期间监测了原发感染情况。通过使用针对编码潜伏膜蛋白1的EBV基因的异源双链追踪分析法，确定了口腔和外周血中EBV毒株的情况。在大多数患者中检测到多种EBV毒株，这些毒株在包括口腔、外周血淋巴细胞和血浆无细胞部分在内的3个不同感染部位持续存在，并可能在这些部位之间传播。我们还追踪了多种毒株从无症状携带者到被诊断为EBV原发感染患者的传播情况。这些数据表明，EBV原发感染情况复杂，存在多种毒株的传播，且不同部位毒株的相对丰度存在明显差异。
