Menotta Michele, Gioacchini Anna M, Amicucci Antonella, Buffalini Michele, Sisti Davide, Stocchi Vilberto
Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, via Saffi 2, 61029 Urbino (PU), Italy.
Rapid Commun Mass Spectrom. 2004;18(2):206-10. doi: 10.1002/rcm.1314.
Ectomycorrhizae formation represents one of the most significant steps in the truffle life cycle and is determined by a complex molecular signaling between two symbionts. In order to understand the molecular pathway of ectomycorrhiza development, we focused on the signaling interaction between the ectomycorrhizal fungus Tuber borchii Vittad. and the Tilia americana L. plant roots. The medium of a pre-symbiotic (T. americana-T. borchii) in vitro system was analysed by headspace solid-phase microextraction coupled with gas chromatography and mass spectrometry. In total, 73 volatile organic compounds (VOCs) were identified. Twenty-nine of these VOCs were produced only during the interaction phase between the two partners, leading to a hypothesis that these molecules might act as molecular messengers in order to pilot the ectomycorrhizae formation.
外生菌根的形成是块菌生命周期中最重要的步骤之一，它由两种共生体之间复杂的分子信号决定。为了了解外生菌根发育的分子途径，我们重点研究了外生菌根真菌波氏块菌（Tuber borchii Vittad.）与美洲椴（Tilia americana L.）植物根系之间的信号相互作用。利用顶空固相微萃取结合气相色谱和质谱分析法，对共生前（美洲椴-波氏块菌）体外系统的培养基进行了分析。总共鉴定出73种挥发性有机化合物（VOCs）。其中29种VOCs仅在两个共生伙伴的相互作用阶段产生，这引发了一种假设，即这些分子可能作为分子信使来引导外生菌根的形成。
