Ezediokpu Marycolette Ndidi, Krause Katrin, Kunert Maritta, Hoffmeister Dirk, Boland Wilhelm, Kothe Erika
Institute of Microbiology, Microbial Communication, Friedrich Schiller University Jena, Neugasse 25, 07743 Jena, Germany.
Max Planck Institute for Chemical Ecology, Bioorganic Chemistry, Hans-Knöll-Straße 8, 07745 Jena, Germany.
J Fungi (Basel). 2022 May 24;8(6):555. doi: 10.3390/jof8060555.
is an ectomycorrhizal basidiomycete with high host specificity. The slow-growing fungus is able to produce twenty sesquiterpenes, including α-barbatene, sativene, isocaryophyllene, α-cuprenene, β-cedrene, ß-copaene, 4-epi-α-acoradiene, and chamigrene in axenic culture. For the three major compounds, Δ-protoilludene, β-barbatene, and an unidentified oxygenated sesquiterpene ( 218.18), changed production during co-cultivation with the ectomycorrhizal partner tree, , could be shown with distinct dynamics. During the mycorrhizal growth of -, Δ-protoilludene and the oxygenated sesquiterpene appeared at similar times, which warranted further studies of potential biosynthesis genes. In silico analyses identified a putative protoilludene synthesis gene, , as being up-regulated in the mycorrhizal stage, in addition to the previously identified, co-regulated geosmin synthase, . We therefore hypothesize that the sesquiterpene synthase has an important role during mycorrhization, through Δ-protoilludene and/or its accompanied oxygenated sesquiterpene production.
是一种具有高宿主特异性的外生菌根担子菌。这种生长缓慢的真菌能够在无菌培养中产生二十种倍半萜，包括α-巴巴烯、檀香烯、异石竹烯、α-库普烯、β-雪松烯、β-古巴烯、4-表-α-菖蒲二烯和香木兰烯。对于三种主要化合物，Δ-原伊鲁烯、β-巴巴烯和一种未鉴定的含氧倍半萜（218.18），在与外生菌根伴生树共培养期间产量发生了变化，其变化动态明显。在-的菌根生长过程中，Δ-原伊鲁烯和含氧倍半萜出现的时间相似，这值得对潜在的生物合成基因进行进一步研究。计算机分析确定了一个假定的原伊鲁烯合成基因，，在菌根阶段上调，此外还有先前鉴定的、共同调控的土臭素合酶，。因此，我们假设倍半萜合酶在菌根形成过程中通过Δ-原伊鲁烯和/或其伴随的含氧倍半萜的产生发挥重要作用。
