固定化酶介导的细菌溶解
Bacteriolysis by immobilized enzymes.
作者信息
Karube I, Suganuma T, Suzuki S
出版信息
Biotechnol Bioeng. 1977 Mar;19(3):301-9. doi: 10.1002/bit.260190302.
Bacteriolytic enzymes produced by Achromobacter lunatus were immobilized in collagen membrane. Intact bacteria such as Pseudomonas solanacearum, Xanthomonas oryzae, Staphylococcus aureus, and Pseudomonas aeruginosa were lyzed with the bacteriolytic enzyme-collagen membrane. Relative activity of the bacteriolytic enzyme-collagen membrane against Pseu. solanacearum was about 2% of that of native bacteriolytic enzymes. No difference in the optimum pH was observed between immobilized enzymes and native enzymes. The bacteriolytic enzymes in the collagen membrane were stable against sodium chloride which was an inhibitor of the native bacteriolytic enzymes. Xanthomonas oryzae and Pseu. aeruginosa were continuously lyzed by a reactor containing the rolled bacteriolytic enzyme-collagen membrane.
无色杆菌产生的溶菌酶被固定在胶原膜中。完整的细菌，如青枯假单胞菌、水稻黄单胞菌、金黄色葡萄球菌和铜绿假单胞菌，被溶菌酶 - 胶原膜裂解。溶菌酶 - 胶原膜对青枯假单胞菌的相对活性约为天然溶菌酶的2%。固定化酶和天然酶之间未观察到最佳pH值的差异。胶原膜中的溶菌酶对氯化钠稳定，而氯化钠是天然溶菌酶的抑制剂。水稻黄单胞菌和铜绿假单胞菌被含有卷起的溶菌酶 - 胶原膜的反应器持续裂解。
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