Müller H P, Blobel H
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Apr;264(1-2):41-8. doi: 10.1016/s0176-6724(87)80123-2.
Staphylococcus hyicus, strain CCM 2368, produced a bacteriolytic enzyme, highly effective on streptococci, some staphylococci and micrococci. Production of the enzyme increased significantly following addition of NaCl (at 1.5%) to the growth medium. The bacteriolytic enzyme could be precipitated from the cell-free staphylococcal culture supernatant with ammonium sulfate at 60% saturation and partially purified by DEAE chromatography. Subsequent isoelectric focusing resulted in complete removal of proteolytic activity and revealed 2 bacteriolytic fractions. One of these (LE IIIa) had an isoelectric point near pH 9.8, the other (LE IIIb) near pH 10.3. LE IIIa lyzed more effectively streptococcal and LE IIIb micrococcal test cells. HPLC gel permeation chromatography also yielded 2 bacteriolytic fractions. The isolated staphylococcal enzyme appeared to be suitable for lysis of streptococci and solubilization of their cell wall structures.
猪葡萄球菌CCM 2368菌株产生一种溶菌酶，对链球菌、一些葡萄球菌和微球菌有高效作用。在生长培养基中添加1.5%的氯化钠后，该酶的产量显著增加。溶菌酶可从无细胞葡萄球菌培养上清液中用60%饱和度的硫酸铵沉淀，并通过DEAE色谱法进行部分纯化。随后的等电聚焦完全去除了蛋白水解活性，并揭示了2个溶菌组分。其中一个（LE IIIa）的等电点接近pH 9.8，另一个（LE IIIb）接近pH 10.3。LE IIIa对链球菌测试细胞的裂解效果更有效，而LE IIIb对微球菌测试细胞的裂解效果更有效。HPLC凝胶渗透色谱法也产生了2个溶菌组分。分离出的葡萄球菌酶似乎适用于链球菌的裂解及其细胞壁结构的溶解。
