Chtcheglova Lilia A, Shubeita George T, Sekatskii Sergey K, Dietler Giovanni
Laboratoire de Physique de la Matière Vivante, Institut de Physique de la Matière Complexe, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
Biophys J. 2004 Feb;86(2):1177-84. doi: 10.1016/S0006-3495(04)74192-2.
A new method of direct and continuous measurement of the spring constant of single molecule or molecular complex is elaborated. To that end the standard force spectroscopy technique with functionalized tips and samples is combined with a small dithering of the tip. The change of the dithering amplitude as a function of the pulling force is measured to extract the spring constant of the complex. The potentialities of this method are illustrated for the experiments with single bovine serum albumin-its polyclonal antibody (Ab-BSA) and fibrinogen-fibrinogen complexes.
阐述了一种直接连续测量单分子或分子复合物弹簧常数的新方法。为此，将具有功能化尖端和样品的标准力谱技术与尖端的小幅抖动相结合。测量抖动幅度随拉力的变化，以提取复合物的弹簧常数。通过单牛血清白蛋白-其多克隆抗体（Ab-BSA）和纤维蛋白原-纤维蛋白原复合物的实验说明了该方法的潜力。
