Institute of Biomedical Chemistry, 10, Pogodinskaya St., 119121 Moscow, Russia.
Int J Mol Sci. 2018 Apr 10;19(4):1142. doi: 10.3390/ijms19041142.
This review is focused on the atomic force microscopy (AFM) capabilities to study the properties of protein biomolecules and to detect the proteins in solution. The possibilities of application of a wide range of measuring techniques and modes for visualization of proteins, determination of their stoichiometric characteristics and physicochemical properties, are analyzed. Particular attention is paid to the use of AFM as a molecular detector for detection of proteins in solutions at low concentrations, and also for determination of functional properties of single biomolecules, including the activity of individual molecules of enzymes. Prospects for the development of AFM in combination with other methods for studying biomacromolecules are discussed.
本文重点介绍原子力显微镜（AFM）在研究蛋白质生物分子性质和检测溶液中蛋白质方面的应用。分析了广泛应用各种测量技术和模式来可视化蛋白质、确定其化学计量特征和物理化学性质的可能性。特别关注了将 AFM 用作分子探测器，以检测低浓度溶液中的蛋白质，以及用于测定单个生物分子的功能特性，包括酶的单个分子的活性。讨论了 AFM 与其他生物大分子研究方法结合发展的前景。
