Smirnova Svetlana V, Torocheshnikova Irina I, Formanovsky Andrey A, Pletnev Igor V
Division of Analytical Chemistry, Chemical Department, Lomonosov Moscow State University, Leninskie Gory, 119992, Moscow, Russia.
Anal Bioanal Chem. 2004 Mar;378(5):1369-75. doi: 10.1007/s00216-003-2398-8. Epub 2004 Jan 28.
Amino acids Trp, Gly, Ala, Leu are extracted efficiently from aqueous solution at pH 1.5-4.0 (Lys and Arg at pH 1.5-5.5) into the room temperature ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (BmimPF(6)) with dicyclohexano-18-crown-6 (CE). The most hydrophilic amino acids such as Gly are extracted as efficiently as the less hydrophilic (92-96%). The influence of pH, amino acid and crown ether concentration, volume ratio of aqueous and organic phases, and presence of some cations on amino acid recovery were studied. The ratio of amino acid to crown ether in the extracted species is 1:1 for cationic Trp, Leu, Ala, and Gly and to 1:2 for dicationic Arg and Lys. This ionic liquid extraction system was used successfully for the recovery of amino acids from pharmaceutical samples and fermentation broth, and was followed by fluorimetric determination.
在pH值为1.5 - 4.0（赖氨酸和精氨酸在pH值为1.5 - 5.5）的条件下，色氨酸、甘氨酸、丙氨酸、亮氨酸能从水溶液中被高效萃取到室温离子液体1-丁基-3-甲基咪唑六氟磷酸盐（BmimPF₆）中，萃取剂为二环己基-18-冠-6（CE）。亲水性最强的氨基酸如甘氨酸，其萃取效率与亲水性较弱的氨基酸相当（92 - 96%）。研究了pH值、氨基酸和冠醚浓度、水相和有机相体积比以及某些阳离子的存在对氨基酸回收率的影响。对于阳离子型的色氨酸、亮氨酸、丙氨酸和甘氨酸，被萃取物种中氨基酸与冠醚的比例为1:1；对于双阳离子型的精氨酸和赖氨酸，该比例为1:2。此离子液体萃取体系成功用于从药物样品和发酵液中回收氨基酸，并随后进行荧光测定。
