Ahmed Yomna M, Badawy Sayed S, Abdel-Haleem Fatehy M
Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
Center for Hazards Mitigation, Environmental Studies and Research (CHMESR), Cairo University, Giza, Egypt.
Microchem J. 2022 Jun;177:107276. doi: 10.1016/j.microc.2022.107276. Epub 2022 Feb 10.
Daclatasvir dihydrochloride (DAC) is an anti-hepatitis C virus (HCV) drug that has recently proven to be a promising candidate for the treatment of SARS-CoV-2. Still, there is a lack of sensitive potentiometric methods for its determination. In this work, carbon paste sensors based on dibenzo-18-crown-6 (DB18C6) were fabricated and optimized for the sensitive and selective potentiometric determination of DAC in Daclavirocyrl® tablets, serum, and urine samples. The best performance was obtained by two sensors referred to as sensor I and sensor II. Both sensors exhibited a wide linear response range of 5×10 - 1×10 mol/L, and Nernstian slopes of 29.8 ± 1.18 and 29.5 ± 1.00 mV/decade, with limits of detection, 4.8×10 and 3.2×10 mol/L, for the sensors I and II, respectively. Sensors I and II displayed fast response times of 5-8 and 5-6 s, respectively, with great reversibility and no memory effect. Moreover, the sensors exhibited a lifetime of 16 days. For the study of sensors morphology and elucidation of the interaction mechanism, the scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (H NMR) techniques were performed. A selectivity study was performed, and the proposed sensors exhibited good discrimination between DAC and potentially coexisting interferents with sensor II displaying better selectivity. Finally, sensor II was successfully applied for the determination of DAC in the above-mentioned samples, with recovery values ranging from 99.25 to 101.42%, and relative standard deviation (RSD) values ranging from 0.79 to 1.53% which reflected the high accuracy and precision.
盐酸达卡他韦（DAC）是一种抗丙型肝炎病毒（HCV）药物，最近已被证明是治疗严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的有前景的候选药物。然而，目前仍缺乏用于其测定的灵敏电位法。在本工作中，制备了基于二苯并-18-冠-6（DB18C6）的碳糊传感器，并对其进行了优化，用于灵敏且选择性地电位测定达卡他韦片、血清和尿液样品中的DAC。两个被称为传感器I和传感器II的传感器表现出最佳性能。两个传感器均呈现出5×10 - 1×10 mol/L的宽线性响应范围，能斯特斜率分别为29.8 ± 1.18和29.5 ± 1.00 mV/十倍浓度，传感器I和II的检测限分别为4.8×10和3.2×10 mol/L。传感器I和II的响应时间分别为5 - 8秒和5 - 6秒，响应速度快，具有良好的可逆性且无记忆效应。此外，传感器的寿命为16天。为了研究传感器的形态并阐明相互作用机制，进行了扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和核磁共振（H NMR）技术研究。进行了选择性研究，所提出的传感器在DAC与潜在共存干扰物之间表现出良好的区分能力，其中传感器II表现出更好的选择性。最后，传感器II成功应用于上述样品中DAC的测定，回收率在99.25%至101.42%之间，相对标准偏差（RSD）值在0.79%至1.53%之间（反映了高准确度和精密度）。
