抗磷脂酰丝氨酸抗体在培养中影响大鼠卵黄囊：抗磷脂综合征中胎儿丢失的一种机制。
Antiphosphatidylserine antibodies affect rat yolk sacs in culture: a mechanism for fetal loss in antiphospholipid syndrome.
作者信息
Matalon Shelly Tartakover, Shoenfeld Yehuda, Blank Miri, Yacobi Sarah, von Landenberg Philipp, Ornoy Asher
机构信息
Department of Anatomy and Cell Biology, Laboratory of Teratology, Hebrew University Hadassah Medical School, Jerusalem.
出版信息
Am J Reprod Immunol. 2004 Feb;51(2):144-51. doi: 10.1046/j.8755-8920.2003.00140.x.
PROBLEM
A variety of reproductive impairments have been reported in the context of the antiphospholipid syndrome (APS). APS is associated with the presence of antibodies to negatively charged phospholipids that may affect the outcome of pregnancy.
METHOD OF STUDY
Rat embryos were cultured within their yolk sacs. The effects of two antiphosphatidylserine monoclonal aPS antibodies (HL5B, RR7F) regarding their influence on growth and apoptotic events of the yolk sacs, as well as on growth and the morphology of the embryos, were studied.
RESULTS
Exposure of rat embryos and their yolk sacs to aPS inhibited yolk sac growth. Moreover, increased number of apoptotic events of giant cells in the aPS-exposed ectoplacental cone was found in comparison with control IgG-exposed giant cells (P < 0.05). No significant damage was observed in the embryos.
CONCLUSIONS
The results suggest that aPS affect growth and apoptosis of rat ectoplacental cone.
问题
在抗磷脂综合征（APS）的背景下，已经报道了多种生殖功能障碍。APS与针对带负电荷磷脂的抗体的存在有关，这些抗体可能会影响妊娠结局。
研究方法
将大鼠胚胎培养在其卵黄囊中。研究了两种抗磷脂酰丝氨酸单克隆aPS抗体（HL5B、RR7F）对卵黄囊生长和凋亡事件以及对胚胎生长和形态的影响。
结果
将大鼠胚胎及其卵黄囊暴露于aPS会抑制卵黄囊生长。此外，与暴露于对照IgG的巨细胞相比，在暴露于aPS的外胎盘锥体中发现巨细胞凋亡事件的数量增加（P < 0.05）。在胚胎中未观察到明显损伤。
结论
结果表明aPS会影响大鼠外胎盘锥体的生长和凋亡。
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