Chin-Ying Stephen Hsu, Xiaoli Gao, Jisheng Pan, Wefel James S
Department of Preventive Dentistry, Faculty of Dentistry, National University of Singapore, 5 Lower Kent Ridge Road, 119074, Singapore, Singapore.
J Dent. 2004 Feb;32(2):161-7. doi: 10.1016/j.jdent.2003.10.004.
The objective of this study was to investigate the effects of CO(2) laser on fluoride uptake in the loosely- and firmly-bound forms in enamel.
Five human molars were cut into halves before being treated with 2.0% NaF topical gel. Each half had three windows on the enamel surface, including one control and two experimental windows irradiated by two laser therapies. One half of each tooth was treated with 1 M KOH solution to remove the loosely-bound fluoride (calcium fluoride). A tooth section was obtained from each window and the relative fluorine concentration was analyzed with Secondary Ion Mass Spectrometry (SIMS). The morphology of the enamel surfaces in the windows was examined using an Environmental Scanning Electron Microscope (ESEM).
Significant laser-induced increases in the uptake of fluoride were revealed in both loosely-bound and firmly-bound apatitic fluoride, with both laser treatments (all p<0.001). Calcium fluoride-like deposits on the enamel surfaces receiving the combined laser-fluoride treatment were revealed by ESEM.
The finding of this study substantiated the laser effect in increasing the fluoride uptake into enamel.
本研究的目的是调查二氧化碳激光对釉质中松散结合和紧密结合形式氟摄取的影响。
五颗人类磨牙在接受2.0%氟化钠局部凝胶治疗前被切成两半。每一半在釉质表面有三个窗口，包括一个对照窗口和两个接受两种激光疗法照射的实验窗口。每颗牙齿的一半用1M氢氧化钾溶液处理以去除松散结合的氟（氟化钙）。从每个窗口获取牙齿切片，并用二次离子质谱（SIMS）分析相对氟浓度。使用环境扫描电子显微镜（ESEM）检查窗口中釉质表面的形态。
两种激光治疗均显示，激光诱导的松散结合和紧密结合的磷灰石氟摄取均显著增加（所有p<0.001）。ESEM显示，接受激光 - 氟联合治疗的釉质表面有类似氟化钙的沉积物。
本研究结果证实了激光在增加氟进入釉质摄取方面的作用。
