Abeyta Michael J, Clark Amander T, Rodriguez Ryan T, Bodnar Megan S, Pera Renee A Reijo, Firpo Meri T
Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California at San Francisco, 94143-0546, USA.
Hum Mol Genet. 2004 Mar 15;13(6):601-8. doi: 10.1093/hmg/ddh068. Epub 2004 Jan 28.
Human embryonic stem cells (hESCs) have the potential to differentiate to diverse cell types. This ability endows hESCs with promise for the development of novel therapeutics, as well as promise for the development of a rigorous genetic system to probe human gene function. However, in spite of the impending utility of hESCs for clinical and basic applications, little is known about their fundamental properties. Recent reports have documented transcriptional profiles of mouse embryonic stem cells (mESCs), adult stem cells and a single hESC line, H9. To date, however, the transcriptional profiles of independently-derived hESC lines have not been compared. In order to examine the similarities and differences in multiple hESC lines, we compared gene expression profiles of the HSF-1, HSF-6 and H9 lines. We found that the majority of genes examined were expressed in all three cell lines. However, we also observed that each line possessed a unique expression signature; the expression of many genes was limited to just one or two hESC lines. We suggest that the observed differences in gene expression between independently-derived hESC lines may reflect inherent differences in the initial culture of each line and/or the underlying genetics of the embryos from which the lines were derived.
人类胚胎干细胞（hESCs）具有分化为多种细胞类型的潜力。这种能力赋予了hESCs开发新型疗法的前景，以及开发一个严格的遗传系统来探究人类基因功能的前景。然而，尽管hESCs在临床和基础应用方面即将发挥效用，但人们对其基本特性知之甚少。最近的报告记录了小鼠胚胎干细胞（mESCs）、成体干细胞和单一hESC系H9的转录谱。然而，迄今为止，尚未对独立来源的hESC系的转录谱进行比较。为了研究多个hESC系之间的异同，我们比较了HSF - 1、HSF - 6和H9系的基因表达谱。我们发现，所检测的大多数基因在所有三个细胞系中均有表达。然而，我们也观察到每个细胞系都具有独特的表达特征；许多基因的表达仅限于一两个hESC系。我们认为，独立来源的hESC系之间观察到的基因表达差异可能反映了每个细胞系初始培养中的固有差异和/或这些细胞系所源自胚胎的潜在遗传学差异。
