Butcher P J
Med Hypotheses. 1992 Nov;39(3):275-80. doi: 10.1016/0306-9877(92)90122-s.
Epidemiological data has suggested the involvement of calcium metabolism in the incidence of multiple sclerosis through a link with high childhood milk intake followed by a large or sudden reduction during the adolescent growth spurt. In this study polyacrylamide gel electrophoresis of SDS-solubilized proteins in the aqueous insoluble pellet fractions of laboratory mouse brain tissue at 6 months of age has shown that a dietary reduction from high calcium intake to 0.005% CaCO3 or less in early life can cause a change in the electrophoretic pattern of low molecular weight proteins. This change is similar in appearance and molecular weight to an abnormality seen in the electrophoretic pattern of SDS-solubilized proteins in the aqueous insoluble pellet fractions of multiple sclerosis plaque. It is proposed that this finding could indicate a worthwhile area for future research and that the hypothesis of a reduction from high to very low calcium intake during adolescence being involved in the pathogenesis of multiple sclerosis should not be discounted.
流行病学数据表明，钙代谢与多发性硬化症的发病有关，其关联在于儿童期牛奶摄入量高，随后在青春期生长突增期间大幅或突然减少。在本研究中，对6月龄实验室小鼠脑组织水不溶性沉淀部分中SDS溶解蛋白进行聚丙烯酰胺凝胶电泳显示，生命早期从高钙摄入量降至0.005%碳酸钙或更低的饮食会导致低分子量蛋白质电泳图谱发生变化。这种变化在外观和分子量上与多发性硬化症斑块水不溶性沉淀部分中SDS溶解蛋白电泳图谱中出现的异常相似。有人提出，这一发现可能表明是未来研究的一个有价值的领域，而且青春期从高钙摄入量降至极低钙摄入量参与多发性硬化症发病机制的假说不应被忽视。
