人羊膜上皮细胞和间充质细胞的体外损伤修复
In vitro lesion repair by human amnion epithelial and mesenchymal cells.
作者信息
Bilic Grozdana, Ochsenbein-Kölble Nicole, Hall Heike, Huch Renate, Zimmermann Roland
机构信息
Department of Obstetrics, Unit of Perinatal Physiology, Zurich University Hospital, Switzerland.
出版信息
Am J Obstet Gynecol. 2004 Jan;190(1):87-92. doi: 10.1016/j.ajog.2003.07.011.
OBJECTIVE
The purpose of this study was to compare wound healing by human amnion epithelial and mesenchymal cells from preterm and term placenta with the use of an in vitro lesion repair assay.
STUDY DESIGN
Lesions were created in confluent monolayers of amnion epithelial and mesenchymal cells from preterm and term placentas. The repair was monitored by the measurement of the lesion area and the response to potential stimulants (platelet-derived growth factor, tumor necrosis factor-alpha, fibrinogen, and phorbol myristate acetate). Cell proliferation was detected with 5-bromodeoxyuridine staining.
RESULTS
Lesion repair was complete within 40 hours in control epithelial cultures from preterm and term placenta but incomplete in mesenchymal cultures (preterm cells, 80%; term cells, 40%). Platelet-derived growth factor, tumor necrosis factor-alpha, fibrinogen, and phorbol myristate acetate did not accelerate repair in either cell type.
CONCLUSION
An in vitro lesion repair assay revealed the differences in lesion repair capacity between amnion epithelial and mesenchymal cells and between mesenchymal cells from preterm and term placenta.
目的
本研究的目的是通过体外损伤修复试验，比较来自早产和足月胎盘的人羊膜上皮细胞和间充质细胞的伤口愈合情况。
研究设计
在来自早产和足月胎盘的羊膜上皮细胞和间充质细胞的汇合单层中制造损伤。通过测量损伤面积和对潜在刺激物（血小板衍生生长因子、肿瘤坏死因子-α、纤维蛋白原和佛波醇肉豆蔻酸酯）的反应来监测修复情况。用5-溴脱氧尿苷染色检测细胞增殖。
结果
来自早产和足月胎盘的对照上皮培养物中的损伤修复在40小时内完成，但间充质培养物中的修复不完全（早产细胞，80%；足月细胞，40%）。血小板衍生生长因子、肿瘤坏死因子-α、纤维蛋白原和佛波醇肉豆蔻酸酯均未加速任何一种细胞类型的修复。
结论
体外损伤修复试验揭示了羊膜上皮细胞和间充质细胞之间以及早产和足月胎盘间充质细胞之间损伤修复能力的差异。
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