Colvin G A, Lambert J-F, Abedi M, Hsieh C-C, Carlson J E, Stewart F M, Quesenberry P J
Department of Research, Roger Williams Medical Center, Providence, RI, USA.
Leukemia. 2004 Mar;18(3):575-83. doi: 10.1038/sj.leu.2403268.
In unperturbed mice, the marrow cell numbers correlate with the stem cell numbers. High levels of long-term marrow engraftment are obtained with infusion of high levels of marrow cells in untreated mice. To address the issue of stem cell competition vs 'opening space', knowledge of total murine marrow cellularity and distribution of stem and progenitor cells are necessary. We determined these parameters in different mouse strains. Total cellularity in BALB/c mice was 530+/-20 million cells; stable from 8 weeks to 1 year of age. C57BL/6J mice had 466+/-48 million marrow cells. Using these data, theoretical models of infused marrow (40 million cells) replacing or adding to host marrow give chimerism values of 7.5 and 7.0%, respectively; the observed 8-week engraftment of 40 million male BALB/c marrow cells into female hosts (72 mice) gave a value of 6.91+/-0.4%. This indicates that syngeneic engraftment is determined by stem cell competition. Our studies demonstrate that most marrow cells, progenitors and engraftable stem cells are in the spine. There was increased concentration of progenitors in the spine. Total marrow harvest for stem cell purification and other experimental purposes was both mouse and cost efficient with over a four-fold decrease in animal use and a financial saving.
在未受干扰的小鼠中，骨髓细胞数量与干细胞数量相关。在未经处理的小鼠中，通过输注高水平的骨髓细胞可实现高水平的长期骨髓植入。为了解决干细胞竞争与“开辟空间”的问题，了解小鼠骨髓细胞总数以及干细胞和祖细胞的分布情况是必要的。我们在不同的小鼠品系中测定了这些参数。BALB/c小鼠的细胞总数为530±2000万个细胞；在8周龄至1岁时保持稳定。C57BL/6J小鼠有466±4800万个骨髓细胞。利用这些数据，输注的骨髓（4000万个细胞）替代或添加到宿主骨髓中的理论模型分别给出了7.5%和7.0%的嵌合率；观察到将4000万个雄性BALB/c骨髓细胞输注到雌性宿主（72只小鼠）中8周后的植入率为6.91±0.4%。这表明同基因植入是由干细胞竞争决定的。我们的研究表明，大多数骨髓细胞、祖细胞和可植入的干细胞都在脊柱中。脊柱中的祖细胞浓度增加。为了干细胞纯化和其他实验目的进行的全骨髓采集在小鼠使用和成本方面都很高效，动物使用量减少了四倍以上，还节省了资金。
