Ema Hideo
State Key Laboratory of Experimental Hematology, National Clinical Research Center for Hematological Disorders, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
Blood Sci. 2019 Sep 17;1(1):116-118. doi: 10.1097/BS9.0000000000000006. eCollection 2019 Aug.
Ex vivo expansion of hematopoietic stem cells (HSCs) is considered the holy grail in stem cell biology and therapy, as it has long been difficult to make this procedure possible. Yamazaki's research team has established new, polyvinyl alcohol-based culture conditions and shown a significant expansion of mouse HSCs from a small number of cells after a month of culture. Surprisingly, expanded HSCs were able to reconstitute unconditioned normal mice. There is generally a technical concern in limiting dilution assay to estimate a fold-expansion of HSCs. But, this work paves the way toward expansion of human HSCs useful for transplantation medicine.
造血干细胞（HSCs）的体外扩增被认为是干细胞生物学和治疗领域的圣杯，因为长期以来一直难以实现这一过程。山崎的研究团队建立了基于聚乙烯醇的新培养条件，并表明在培养一个月后，少量小鼠造血干细胞能实现显著扩增。令人惊讶的是，扩增后的造血干细胞能够重建未经预处理的正常小鼠。在通过有限稀释法评估造血干细胞的扩增倍数时，通常存在技术上的问题。但是，这项工作为扩增对移植医学有用的人类造血干细胞铺平了道路。
