一种用于通过聚合酶链反应使用新型寡核苷酸探针诊断卡氏肺孢子虫肺炎的替代DNA提取方法。

An alternative to DNA extraction for the diagnosis of Pneumocystis carinii pneumonia by polymerase chain reaction using a new oligonucleotide probe.

作者信息

Borensztein L, Hatin I, Simonpoli A M, Ugarte E, Girard P M, Jaureguiberry G

机构信息

INSERM U.13, Hôpital Claude Bernard, Paris, France.

出版信息

Mol Cell Probes. 1992 Oct;6(5):361-5. doi: 10.1016/0890-8508(92)90028-v.

DOI:10.1016/0890-8508(92)90028-v

PMID:1474976

Abstract

We have used new specific primers and probe in a polymerase chain reaction (PCR) followed by Southern blot assays to detect Pneumocystis carinii in human bronchoalveolar lavage samples obtained from HIV-infected patients with pulmonary symptoms. To facilitate the procedure we developed a filtration technique without DNA extraction yielding a high sensitivity (18/18 positive results). The high specificity of the technique was shown by testing immunosuppressed patients without P. carinii pneumonia.

摘要

我们使用新型特异性引物和探针进行聚合酶链反应（PCR），随后进行Southern印迹分析，以检测从有肺部症状的HIV感染患者获取的人支气管肺泡灌洗样本中的卡氏肺孢子虫。为便于操作，我们开发了一种无需DNA提取的过滤技术，其灵敏度很高（18/18例阳性结果）。通过对无卡氏肺孢子虫肺炎的免疫抑制患者进行检测，显示了该技术的高特异性。