使用半定量聚合酶链反应评估大鼠模型中卡氏肺孢子虫感染的发病及治疗情况。
Use of semiquantitative PCR to assess onset and treatment of Pneumocystis carinii infection in rat model.
作者信息
O'Leary T J, Tsai M M, Wright C F, Cushion M T
机构信息
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, D.C. 20306-6000, USA.
出版信息
J Clin Microbiol. 1995 Mar;33(3):718-24. doi: 10.1128/jcm.33.3.718-724.1995.
The use of semiquantitative PCR (SQPCR) to assess Pneumocystis carinii pneumonia (PCP) infection and its response to treatment was studied with rats. Groups of eight rats were immunosuppressed with steroids for 3 to 12 weeks. Untreated controls were maintained for the same periods. Three groups of rats were treated with pentamidine, three groups were treated with trimethoprim-sulfamethoxazole, and three groups of rats were tapered from steroids. At various times during suppression, rats from the different groups were sacrificed. At necropsy, lungs were lavaged to obtain bronchoalveolar fluids and then homogenized. Bronchoalveolar fluids and homogenates were assayed by cyst counting and SQPCR. An increase in the SQPCR signal was seen throughout immunosuppression, with a slow decrease upon the withdrawal of steroids and a faster decrease with drug treatment. SQPCR results with lung homogenates and bronchoalveolar fluids strongly correlated with each other and with cyst counts. These results warrant investigation of SQPCR for assessing treatment results of human P. carinii pneumonia infection.
利用大鼠研究了采用半定量聚合酶链反应（SQPCR）评估卡氏肺孢子虫肺炎（PCP）感染及其对治疗反应的情况。将八只大鼠分为一组，用类固醇进行免疫抑制3至12周。未治疗的对照组维持相同时间。三组大鼠用喷他脒治疗，三组用甲氧苄啶-磺胺甲恶唑治疗，三组大鼠逐渐减少类固醇用量。在免疫抑制期间的不同时间，处死不同组的大鼠。尸检时，对肺进行灌洗以获取支气管肺泡灌洗液，然后进行匀浆。通过囊泡计数和SQPCR对支气管肺泡灌洗液和匀浆进行检测。在整个免疫抑制过程中，SQPCR信号增加，停用类固醇后缓慢下降，药物治疗后下降更快。肺匀浆和支气管肺泡灌洗液的SQPCR结果彼此之间以及与囊泡计数密切相关。这些结果值得对SQPCR用于评估人类卡氏肺孢子虫肺炎感染的治疗结果进行研究。
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