Cho Jungsook, Lee Hyeong-Kyu
College of Medicine, Dongguk University, Gyeongju, Gyeongbuk 780-714, South Korea.
Eur J Pharmacol. 2004 Feb 6;485(1-3):105-10. doi: 10.1016/j.ejphar.2003.11.064.
The present study evaluated effects of wogonin (5,7-dihydroxy-8-methoxyflavone) on excitotoxic and oxidative stress-induced neuronal damage in primary cultured rat cortical cells. Wogonin was shown to inhibit the excitotoxicity induced by glutamate or N-methyl-D-aspartic acid, whereas it showed no effects on the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid- or kainate-induced toxicity. In addition, wogonin inhibited the oxidative neuronal damage induced by H(2)O(2), xanthine/xanthine oxidase, and by a glutathione depleting agent D,L-buthionine [S,R]-sulfoximine. Furthermore, wogonin dramatically inhibited lipid peroxidation initiated by Fe(2+) and L-ascorbic acid in rat brain homogenates. It also exhibited 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Taken together, these results demonstrate that wogonin exhibits neuroprotective actions in cultured cortical cells by inhibiting excitotoxicity and various types of oxidative stress-induced damage, and that its antioxidant actions with radical scavenging activity may contribute, at least in part, to the neuroprotective effects.
本研究评估了汉黄芩素（5,7 - 二羟基 - 8 - 甲氧基黄酮）对原代培养的大鼠皮质细胞中兴奋性毒性和氧化应激诱导的神经元损伤的影响。结果表明，汉黄芩素可抑制由谷氨酸或N - 甲基 - D - 天冬氨酸诱导的兴奋性毒性，而对α - 氨基 - 3 - 羟基 - 5 - 甲基 - 4 - 异恶唑丙酸或海人藻酸诱导的毒性无影响。此外，汉黄芩素可抑制由过氧化氢、黄嘌呤/黄嘌呤氧化酶以及谷胱甘肽消耗剂D，L - 丁硫氨酸 - [S，R] - 亚砜亚胺诱导的氧化性神经元损伤。此外，汉黄芩素可显著抑制大鼠脑匀浆中由亚铁离子和L - 抗坏血酸引发的脂质过氧化反应。它还表现出1,1 - 二苯基 - 2 - 苦基肼自由基清除活性。综上所述，这些结果表明，汉黄芩素通过抑制兴奋性毒性和各种类型的氧化应激诱导的损伤，在培养的皮质细胞中表现出神经保护作用，并且其具有自由基清除活性的抗氧化作用可能至少部分地促成了神经保护作用。
