Institute of Bioresources and Sustainable Development (An Autonomous Institute under the Department of Biotechnology, Goverment of India), Takyelpat, Imphal 795001, Manipur, India.
Department of Zoology, Manipur University (MU), Imphal 795003, Manipur, India.
Molecules. 2023 Mar 23;28(7):2901. doi: 10.3390/molecules28072901.
Retz. (Fam. Combretaceae), locally called Manahei, is a well-known medicinal plant that grows wildly in Manipur, a Northeastern state of India. It is used as a mild laxative, an anti-inflammatory agent, and a remedy for piles, colds, and ulcers by ethnic communities of the state. The hydroalcoholic extract obtained from four fruit samples of collected from different locations in Manipur were analyzed using gas chromatography-mass spectrometry (GC-MS) and high-performance thin-layer chromatography (HPTLC) for their chemical constituents and evaluated for their anticancer activity against the colon cancer cell HCT 116. GC-MS analysis results indicated significant variation in the composition and percentage of major compounds present in the extracts. 1,2,3-Benzenetriol was the most abundant chemical constituent present in all four extracts of , ranging from 20.95 to 43.56%. 2-Cyclopenten-1-one, 5-hydroxymethylfurfural, and catechol were commonly present in all extracts. Two marker compounds, gallic acid and ellagic acid, were also quantified usingHPTLC in all four extracts of . The highest content of gallic acid (22.44 ± 0.056 µg/mg of dried extract) was observed in TCH, and that of ellagic acidwas found in TYH (11.265 ± 0.089 µg/mg of dried extract). The IC value of TYH for the DPPH and ABTS assays (12.16 ± 0.42 and 7.80 ± 0.23 µg/mL) was found to be even lower than that of Trolox (18 ± 0.44 and 10.15 ± 0.24 µg/mL), indicating its strong antioxidant properties among the four extracts of . The MTT assay determined the effect of extracts on the viability of HCT 116 cells. TYH showed the highest activity with anIC value of 52.42 ± 0.87 µg/mL, while the lowest activity was observed in TCH (172.05 ± 2.0 µg/mL). The LDH assay confirmed the cytotoxic effect of TYH in HCT 116 cells. TYH was also found to induce caspase-dependent apoptosis in HCT 116 cells after 48 h of treatment. Our study provides insight into the diversity of in Manipur and its potential activity against colon cancer.
瑞茨（Combretaceae 科），当地称为 Manahei，是一种生长在印度东北部曼尼普尔邦的著名药用植物。它被该邦的少数民族用作温和的泻药、消炎药和治疗痔疮、感冒和溃疡的药物。从曼尼普尔邦不同地点收集的四个果实样本中获得的水醇提取物，使用气相色谱-质谱联用（GC-MS）和高效薄层色谱（HPTLC）进行化学成分分析，并评估其对结肠癌细胞 HCT 116 的抗癌活性。GC-MS 分析结果表明，提取物中主要化合物的组成和百分比较大。四种 提取物中最丰富的化学成分为 1,2,3-苯三醇，其含量范围为 20.95-43.56%。2-环戊烯-1-酮、5-羟甲基糠醛和儿茶酚通常存在于所有提取物中。两种标记化合物，没食子酸和鞣花酸，也通过 HPTLC 在四种 提取物中进行了定量。TCH 中没食子酸的含量最高（22.44±0.056μg/mg 干提取物），TYH 中鞣花酸的含量最高（11.265±0.089μg/mg 干提取物）。TYH 在 DPPH 和 ABTS 测定中的 IC 值（12.16±0.42 和 7.80±0.23μg/mL）甚至低于 Trolox（18±0.44 和 10.15±0.24μg/mL），表明其在四种 提取物中的抗氧化性能最强。MTT 测定确定了 提取物对 HCT 116 细胞活力的影响。TYH 表现出最高的活性，IC 值为 52.42±0.87μg/mL，而 TCH 的活性最低（172.05±2.0μg/mL）。LDH 测定证实了 TYH 在 HCT 116 细胞中的细胞毒性作用。TYH 还被发现能诱导 HCT 116 细胞在 48 小时处理后发生 caspase 依赖性凋亡。我们的研究为曼尼普尔邦 多样性及其对结肠癌潜在活性提供了深入了解。
