Darmochwal-Kolarz D, Rolinski J, Buczkowski J, Tabarkiewicz J, Leszczynska-Gorzelak B, Zych I, Oleszczuk J
Department of Obstetrics and Perinatology, University School of Medicine, ul. Jaczewskiego 8, 20 950 Lublin, Poland.
Immunol Lett. 2004 Jan 30;91(1):71-4. doi: 10.1016/j.imlet.2003.11.006.
It has been suggested lately that some types of antigen presenting cells-myeloid dendritic (DC-1) cells can differentiate the immune response towards Th1 type immunity, whereas lymphoid cells (DC-2) can stimulate Th2 type immunity. It has been observed that neonates are deficient in Th1 response. The purpose of our study was to estimate the proportions of immature myeloid (CD1c(+)) and lymphoid (BDCA-2(+), BDCA-4(+)) dendritic cells and the CD1c(+):BDCA-2(+) cell ratio in cord blood of healthy neonates in comparison with dendritic cells of healthy adults. Thirty healthy neonates born from normal pregnancies and 30 healthy adults were included in the study. The dendritic cells were isolated from cord and peripheral blood, stained with anti-CD1c, anti-BDCA-2, anti-BDCA-4, anti-CD123 and anti-CD19 monoclonal antibodies and estimated using flow cytometry. The percentage of CD1c(+) dendritic cells in cord blood of healthy newborns did not differ significantly when compared to those in peripheral blood of healthy adults. The percentages of cord blood BDCA-2(+) and BDCA-4(+) dendritic cells of neonates were significantly lower when compared to lymphoid dendritic cells in peripheral blood of adults. The CD1c(+):BDCA-2(+) ratio was significantly higher in cord blood of neonates in comparison with CD1c(+):BDCA-2(+) ratio in adult's blood. Myeloid and lymphoid dendritic cells may be involved in the immune regulation during fetal development. Immature myeloid dendritic cells are predominant in cord blood of healthy neonates. Immature lymphoid dendritic cells are not the major population of dendritic cells in cord blood.
最近有人提出，某些类型的抗原呈递细胞——髓样树突状（DC-1）细胞可使免疫反应向Th1型免疫分化，而淋巴样细胞（DC-2）可刺激Th2型免疫。据观察，新生儿的Th1反应存在缺陷。我们研究的目的是评估健康新生儿脐带血中未成熟髓样（CD1c(+)）和淋巴样（BDCA-2(+)、BDCA-4(+)）树突状细胞的比例以及CD1c(+):BDCA-2(+)细胞比率，并与健康成年人的树突状细胞进行比较。30例正常妊娠出生的健康新生儿和30例健康成年人纳入本研究。从脐带血和外周血中分离树突状细胞，用抗CD1c、抗BDCA-2、抗BDCA-4、抗CD123和抗CD19单克隆抗体进行染色，并用流式细胞术进行评估。健康新生儿脐带血中CD1c(+)树突状细胞的百分比与健康成年人外周血中的相比，差异无统计学意义。与成年人外周血中的淋巴样树突状细胞相比，新生儿脐带血中BDCA-2(+)和BDCA-4(+)树突状细胞的百分比显著降低。与成年人血液中的CD1c(+):BDCA-2(+)比率相比，新生儿脐带血中的CD1c(+):BDCA-2(+)比率显著更高。髓样和淋巴样树突状细胞可能参与胎儿发育过程中的免疫调节。未成熟髓样树突状细胞在健康新生儿脐带血中占主导地位。未成熟淋巴样树突状细胞不是脐带血中树突状细胞的主要群体。
