Fertig Christiane C, Podczeck Fridrun, Jee Roger D, Smith Mark R
The School of Pharmacy, University of London, 29-39 Brunswick Square, London, WC1N 1AX, UK.
Eur J Pharm Sci. 2004 Feb;21(2-3):155-9. doi: 10.1016/j.ejps.2003.09.011.
Near-infrared (NIR) spectroscopy was used to determine the amylose content in six different starches, whose declared amylose contents ranged from 2 to 95% m/m. The amylose content of starches can vary considerably between batches depending on growth conditions and time of harvesting. An NIR calibration model was developed for amylose using simple laboratory produced mixtures of amylose and amylopectin in different ratios. The spectral region at 1700-1800nm showed a good correlation to the amylose content of these mixtures. A simple absorbance ratio calibration model using standard normal variate and first derivative pre-treated spectra gave a root mean standard error of prediction of 1.2% m/m. Application to real samples gave amylose contents in reasonable agreement with the average values stated by the supplier. NIR spectroscopy provides a rapid and non-destructive method for the quantitative determination and standardisation of amylose in starch and could make a suitable alternative to traditional techniques, such as complex formation of starch with iodine or n-butanol.
近红外（NIR）光谱法用于测定六种不同淀粉中的直链淀粉含量，其宣称的直链淀粉含量范围为2%至95%（质量/质量）。不同批次淀粉的直链淀粉含量会因生长条件和收获时间而有很大差异。利用实验室简单制备的不同比例直链淀粉和支链淀粉混合物，建立了直链淀粉的近红外校准模型。1700 - 1800nm光谱区域与这些混合物的直链淀粉含量显示出良好的相关性。使用标准正态变量和一阶导数预处理光谱的简单吸光度比校准模型，预测的均方根标准误差为1.2%（质量/质量）。应用于实际样品时，得到的直链淀粉含量与供应商声明的平均值相当吻合。近红外光谱法为淀粉中直链淀粉的定量测定和标准化提供了一种快速且无损的方法，可成为传统技术（如淀粉与碘或正丁醇形成络合物）的合适替代方法。
