Brewer C Fred
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Glycoconj J. 2002;19(7-9):459-65. doi: 10.1023/B:GLYC.0000014075.62724.d0.
The carbohydrate binding specificities of the galectin family of animal lectins has been the source of intense recent investigations. Isothermal titration microcalorimetry (ITC) provides direct determination of the thermodynamics of binding of carbohydrates to lectins, and has provided important insights into the fine carbohydrate binding specificities of a wide number of plant and animal lectins. Recent ITC studies have been performed with galectin-1, galectin-3 and galectin-7 and their interactions with sialylated and non-sialylated carbohydrates. The results show important differences in the specificities of these three galectins toward poly-N-acetyllactosamine epitopes found on the surface of cells.
动物凝集素半乳糖凝集素家族的碳水化合物结合特异性一直是近期深入研究的对象。等温滴定量热法（ITC）可直接测定碳水化合物与凝集素结合的热力学过程，并为众多植物和动物凝集素精细的碳水化合物结合特异性提供了重要见解。近期已利用半乳糖凝集素-1、半乳糖凝集素-3和半乳糖凝集素-7开展了ITC研究，以及它们与唾液酸化和非唾液酸化碳水化合物的相互作用。结果表明，这三种半乳糖凝集素对细胞表面发现的多聚N-乙酰乳糖胺表位的特异性存在重要差异。
