Barth Peter J, Ebrahimsade Schokufe, Hellinger Achim, Moll Roland, Ramaswamy Annette
Institute of Pathology, Philipps University Marburg, Baldingerstraße, 35033 Marburg, Germany, Germany.
Department of Surgery, Philipps University Marburg, Marburg, Germany, Germany.
Virchows Arch. 2002 Feb;440(2):128-133. doi: 10.1007/s00428-001-0551-3.
Besides its function as a matrix-producing cell, the CD34+ fibrocyte has been reported to be an antigen-presenting cell capable of priming naive T cells in situ. Therefore, it has been claimed that the CD34+ fibrocyte may play an important role in host response to tissue damage. The objective of the present study was to analyze the presence and distribution of CD34+ fibrocytes and smooth muscle actin (SMA) reactive myofibroblasts in relation to the underlying pancreatic disease. We investigated a total of 12 pancreatic adenocarcinomas, 7 endocrine tumors of the pancreas, and 8 cases of chronic pancreatitis; in 11 cases, normal pancreatic tissue was available. The stroma of normal pancreatic tissue harbored diffusely scattered CD34+ fibrocytes. Chronic pancreatitis was characterized by an increased number of stromal CD34+ fibrocytes paralleled by a gain of SMA reactive myofibroblasts which were not observed in the normal pancreatic stroma. The stroma of pancreatic ductal adenocarcinomas and endocrine tumors was devoid of CD34+ fibrocytes or showed at least a focal loss of this cell type, whereas SMA reactive myofibroblasts were detected in both endocrine tumors and adenocarcinomas. We conclude that detection of CD34+ fibrocytes may constitute an adjunctive tool in distinguishing chronic pancreatitis from ductal adenocarcinoma since the absence of this cell population strongly favors a neoplastic process. Moreover, CD34+ fibrocytes and myofibroblasts appear to be involved in stromal remodeling associated with chronic pancreatitis and ductal adenocarcinoma.
除了作为产生基质的细胞发挥作用外，据报道CD34 +成纤维细胞是一种能够在原位启动幼稚T细胞的抗原呈递细胞。因此，有人认为CD34 +成纤维细胞可能在宿主对组织损伤的反应中发挥重要作用。本研究的目的是分析CD34 +成纤维细胞和平滑肌肌动蛋白（SMA）反应性肌成纤维细胞的存在和分布与潜在胰腺疾病的关系。我们共研究了12例胰腺腺癌、7例胰腺内分泌肿瘤和8例慢性胰腺炎；其中11例有正常胰腺组织。正常胰腺组织的间质中散在分布着CD34 +成纤维细胞。慢性胰腺炎的特征是间质CD34 +成纤维细胞数量增加，同时出现了正常胰腺间质中未观察到的SMA反应性肌成纤维细胞。胰腺导管腺癌和内分泌肿瘤的间质中没有CD34 +成纤维细胞，或至少出现这种细胞类型的局灶性缺失，而在内分泌肿瘤和腺癌中均检测到SMA反应性肌成纤维细胞。我们得出结论，检测CD34 +成纤维细胞可能是区分慢性胰腺炎和导管腺癌的辅助工具，因为这种细胞群体的缺失强烈提示肿瘤形成过程。此外，CD34 +成纤维细胞和肌成纤维细胞似乎参与了与慢性胰腺炎和导管腺癌相关的间质重塑。
