Ljungkvist Göran, Azimi Arman, Mathiasson Lennart
The Sahlgrenska Academy at Göteborg University, Institute of Internal Medicine, Section of Occupational Medicine, St Sigfridsgatan 85, SE-412 66, Göteborg, Sweden.
J Environ Monit. 2004 Feb;6(2):143-6. doi: 10.1039/b311201m. Epub 2004 Jan 16.
A method was developed for the determination of benzene in urine. The sample was pumped through the donor channel of a membrane extraction unit with a micro-porous membrane, separating the donor channel from an identical acceptor channel purged with nitrogen. The analyte reached the acceptor channel by diffusion through the membrane and was then swept by the carrier to a solid sorbent tube, where it was trapped. The analyte was subsequently thermally desorbed and analyzed by gas chromatography (GC) with mass selective detection (MS). After optimization, the recovery was close to quantitative, or 95%. Purging the membrane unit with pure water in between the samples eliminated any memory effects. The linearity was good in the concentration range examined (20-4000 ng l(-1)), with a correlation coefficient of 0.9996. The repeatability at 50 ng l(-1) and 400 ng l(-1) was 1.4% and 1.2%, respectively. The limit of detection was 12 ng l(-1) and the limit of quantification 35 ng l(-1). This enables assessment of benzene exposures of occupationally exposed subjects, of smokers and the majority of the general population. The developed method can be easily automated.
开发了一种测定尿液中苯的方法。样品通过带有微孔膜的膜萃取单元的供体通道泵送，该膜将供体通道与用氮气吹扫的相同受体通道隔开。分析物通过扩散穿过膜到达受体通道，然后被载气吹扫至固体吸附剂管中并被捕集。随后，分析物进行热脱附，并通过带质量选择检测（MS）的气相色谱（GC）进行分析。优化后，回收率接近定量，即95%。在样品之间用纯水吹扫膜单元消除了任何记忆效应。在所研究的浓度范围（20 - 4000 ng l⁻¹）内线性良好，相关系数为0.9996。在50 ng l⁻¹和400 ng l⁻¹时的重复性分别为1.4%和1.2%。检测限为12 ng l⁻¹，定量限为35 ng l⁻¹。这使得能够评估职业暴露人群、吸烟者和大多数普通人群的苯暴露情况。所开发的方法易于自动化。
