Fukuchi T, Sawaguchi S, Hara H, Abe H, Iwata K, Kaiya T
Department of Ophthalmology, Niigata University School of Medicine, Japan.
Nippon Ganka Gakkai Zasshi. 1992 Nov;96(11):1357-65.
Morphological changes of sulfated proteoglycans (S-PGs) in the stroma of alkali injured rabbit corneas were examined electron-histochemically following cuprolinic blue (CB) dye binding. The injured eyes were followed for 2 months by slitlamp microscope and photographs. At 1 hour, 3 days, 1 week, 2 weeks, 1 month, and 2 months after the injury, the eyes were enucleated and studied. After 1 hour, while both collagen fibrils and the filaments of S-PGs had already become disarranged; the number of S-PGs appeared to decrease. From 3 days to 1 week after, the number as well as the electron-density of S-PGs were markedly decreased in all areas. After 2 weeks, CB-positive large filaments appeared in anterior stroma. These became larger, more abnormal in shape, and displayed a higher electron density 1 month later. Normally arranged S-PGs can be seen at 2 months later. It was concluded that S-PGs were diminished in alkali-injured corneas. Once they were replaced by abnormal filaments, they gradually recovered normal morphology. Further more the time course of S-PG change correlated with that of corneal transparency after alkali injury.
采用铜啉蓝（CB）染色结合电子组织化学方法，研究碱烧伤兔角膜基质中硫酸化蛋白聚糖（S-PGs）的形态学变化。用裂隙灯显微镜观察并拍摄伤眼2个月。在伤后1小时、3天、1周、2周、1个月和2个月时摘除眼球进行研究。伤后1小时，胶原纤维和S-PGs的细丝均已紊乱；S-PGs数量似乎减少。伤后3天至1周，所有区域S-PGs的数量和电子密度均显著降低。伤后2周，前基质中出现CB阳性的粗大细丝。1个月后，这些细丝变得更大，形状更异常，电子密度更高。伤后2个月可见排列正常的S-PGs。结果表明，碱烧伤角膜中S-PGs减少。一旦它们被异常细丝取代，它们会逐渐恢复正常形态。此外，S-PG变化的时间进程与碱烧伤后角膜透明度的时间进程相关。
