Fagerholm P, Fitzsimmons T, Härfstrand A, Schenholm M
St Eriks Eye Hospital, Karolinska Institutet, Stockholm, Sweden.
Acta Ophthalmol Suppl (1985). 1992(202):67-72. doi: 10.1111/j.1755-3768.1992.tb02171.x.
The presence and distribution of reactively formed hyaluronic acid (HA) was assessed in the rabbit cornea following a penetrating alkali burn. The injury was inflicted by applying a round, 5.5 mm, filter paper soaked in 1 N NaOH centrally on the cornea for 60 seconds. Biochemical analysis revealed a significant increase of HA two weeks after injury, a peak concentration after 1 month, and a decrease again at three months. Histochemical analysis revealed the presence of hyaluronic acid in the healing epithelium, in the repopulating keratocytes/fibroblasts, and in the cells forming the retrocorneal membrane. Extensive amounts filled lacunae in the stroma as well as the spaces between collagen lamellae. A slow restoration of normal appearing corneal stroma took place at the periphery. Significant staining for HA in lacunae was present centrally in the wound after three months.
在兔角膜穿透性碱烧伤后，评估反应性形成的透明质酸（HA）的存在和分布。通过将浸泡在1 N NaOH中的圆形5.5 mm滤纸置于角膜中央60秒造成损伤。生化分析显示，损伤后两周HA显著增加，1个月时达到峰值浓度，3个月时再次下降。组织化学分析显示，愈合上皮、再生的角膜细胞/成纤维细胞以及形成角膜后膜的细胞中存在透明质酸。大量的透明质酸填充了基质中的腔隙以及胶原板层之间的间隙。周边角膜基质外观缓慢恢复正常。三个月后，伤口中央的腔隙中HA染色明显。
