中国肝细胞癌中的核DNA和线粒体DNA微卫星不稳定性
Nuclear and mitochondrial DNA microsatellite instability in hepatocellular carcinoma in Chinese.
作者信息
Fang Dian-Chun, Fang Li, Wang Rong-Quan, Yang Shi-Ming
机构信息
Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
出版信息
World J Gastroenterol. 2004 Feb 1;10(3):371-5. doi: 10.3748/wjg.v10.i3.371.
AIM
To study the nuclear microsatellite instability (nMSI) at BAT26 and mitochondrial microsatellite instability (mtMSI) in the occurrence and development of hepatocellular carcinoma and the relationship between nMSI and mtMSI.
METHODS
nMSI was observed with PCR and mtMSI with PCR-SSCP in 52 cases of hepatocellular carcinoma.
RESULTS
mtMSI was detected in 11 out of the 52 cases of hepatocellular carcinoma (21.2%). Among the 11 cases of hepatocellular carcinoma with mtMSI, 7 occurred in one locus and 4 in 2 loci. The frequency of mtMSI in the 52 cases of hepatocellular carcinoma showed no correlation to sex, age, infection of hepatitis B, liver cirrhosis as well as positive AFP of the patients (P>0.05). In addition, nMSI was detected in 3 out of 52 cases of hepatocellular carcinoma (5.8%) and there was no correlation of the incidence of mtMSI to that of nMSI (P>0.05).
CONCLUSION
mtMSI may be involved in the coccurrence and development of hepatocellular carcinoma and it is independent of nMSI.
目的
研究BAT26位点的核微卫星不稳定性（nMSI）及线粒体微卫星不稳定性（mtMSI）在肝细胞癌发生发展中的情况以及nMSI与mtMSI之间的关系。
方法
采用PCR法检测52例肝细胞癌患者的nMSI，采用PCR-SSCP法检测mtMSI。
结果
52例肝细胞癌患者中，11例检测到mtMSI（21.2%）。在这11例有mtMSI的肝细胞癌患者中，7例发生在一个位点，4例发生在2个位点。52例肝细胞癌患者中mtMSI的发生率与患者的性别、年龄、乙型肝炎感染、肝硬化以及甲胎蛋白阳性与否均无相关性（P>0.05）。此外，52例肝细胞癌患者中有3例检测到nMSI（5.8%），mtMSI的发生率与nMSI的发生率无相关性（P>0.05）。
结论
mtMSI可能参与肝细胞癌的发生发展，且与nMSI无关。
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