Chang Qing, Liu Zheng-Ren, Wang Da-Yu, Kumar Manoj, Chen Yi-Bei, Qin Ren-Yi
Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
World J Gastroenterol. 2004 Feb 1;10(3):415-8. doi: 10.3748/wjg.v10.i3.415.
To study the role of survivin expression induced by chemotherapy agent (doxorubicin) in the development and anti-chemotherapy of cholangiocarcinoma.
Expression of survivin was detected by SP immunohistochemical technique in 33 cases of cholangiocarcinoma, 28 cases of adjacent noncancerous bile duct, and 5 cases of benign bile duct lesions. Low concentration of doxorubicin (0.05 mg/l) was added in cultured cholangiocarcinoma cell line (QBC939). The expression of survivin was detected by RT-PCR and Western blot at 24 h and 48 h after adding doxorubicin.
Survivin was expressed in 24 of 33 cholangiocarcinoma cases (72.7%). In contrast, no expression of survivin in adjacent noncancerous and benign bile duct lesions was observed (P<0.01). No correlation was found between survivin expression and clinical features. Doxorubicin could markedly (P<0.001) up-regulate survivin mRNA and protein expression of QBC939 cells.
Overexpression of survivin in cholangiocarcinomas may play an important role in the development of cholangiocarcinoma, its relationship with prognosis of cholangiocarcinoma deserves further investigation. Higher expression of survivin is induced by doxorubicin in QBC939. Survivin expression may resist apoptosis induced by chemotherapy agents.
研究化疗药物（阿霉素）诱导的生存素表达在胆管癌发生发展及化疗抵抗中的作用。
采用SP免疫组化技术检测33例胆管癌、28例癌旁非癌性胆管及5例胆管良性病变组织中生存素的表达。在培养的胆管癌细胞系（QBC939）中加入低浓度阿霉素（0.05mg/L），分别于加药后24h和48h采用RT-PCR和Western blot检测生存素的表达。
33例胆管癌组织中有24例（72.7%）生存素呈阳性表达。癌旁非癌性胆管及胆管良性病变组织中均未检测到生存素表达（P<0.01）。生存素表达与胆管癌临床病理特征无相关性。阿霉素能显著上调QBC939细胞生存素mRNA及蛋白表达（P<0.001）。
生存素在胆管癌中高表达可能在胆管癌发生发展中起重要作用，其与胆管癌预后的关系值得进一步研究。阿霉素可诱导QBC939细胞生存素高表达，生存素表达可能是胆管癌细胞化疗抵抗及逃避凋亡的机制之一。
