Zhu Xiao-Dong, Lin Geng-Jin, Qian Li-Ping, Chen Zhong-Qing
Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai, 200040, China.
World J Gastroenterol. 2003 Jul;9(7):1435-8. doi: 10.3748/wjg.v9.i7.1435.
To study the expression of survivin, an inhibitor of apoptosis protein, in human gastric carcinomas and gastric carcinoma models of rats.
With the method of immunohistochemical staining, we studied the expression of survivin in 20 cases of chronic gastritis and 56 cases of gastric carcinomas. We used N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and high dose sodium-chloride diet to induce rat gastric carcinomas. Survivin expression was studied in glandular stomachs of normal rats, adenocarcinomas and tissues adjacent to the tumor, as well as in rats during the induction period.
Survivin was expressed in 27 of 56 (48.2 %) cases of human gastric carcinoma tissues and 1 of 20 (5 %) cases of chronic gastritis. It was found that the expression of survivin had no relation with the elements of age, tumor depth, tumor size, and disease stage, but was significantly related to histological type. The positive rate of survivin expression in cases of intestinal type was significantly higher than that in cases of diffuse type (P<0.05). In animal experiments, survivin expression in glandular stomachs of normal rats, of rats in middle induction period, in adenocarcinomas and tissues adjacent to tumor were 0, 40.0 %, 78.3 % and 38.9 %, respectively. Compared with the survivin expression in normal rats, the differences were significant.
These data imply that survivin plays an important role in the onset of gastric carcinoma and that high survivin expression is an early event of gastric carcinoma.
研究凋亡抑制蛋白survivin在人胃癌组织及大鼠胃癌模型中的表达情况。
采用免疫组化染色法，研究20例慢性胃炎和56例胃癌组织中survivin的表达。用N-甲基-N'-硝基-N-亚硝基胍（MNNG）和高盐饮食诱导大鼠胃癌。研究正常大鼠腺胃、腺癌及癌旁组织以及诱导期大鼠中survivin的表达。
56例人胃癌组织中有27例（48.2%）表达survivin，20例慢性胃炎组织中有1例（5%）表达。发现survivin的表达与年龄、肿瘤深度、肿瘤大小及疾病分期等因素无关，但与组织学类型显著相关。肠型病例中survivin表达阳性率显著高于弥漫型病例（P<0.05）。在动物实验中，正常大鼠腺胃、诱导中期大鼠、腺癌及癌旁组织中survivin的表达分别为0、40.0%、78.3%和38.9%。与正常大鼠中survivin的表达相比，差异有统计学意义。
这些数据表明survivin在胃癌的发生中起重要作用，且survivin高表达是胃癌的早期事件。
