Yang Min, Fang Dian-Chun, Li Qian-Wei, Sun Nian-Xu, Long Qing-Lin, Sui Jian-Feng, Gan Lu
PLA, Research and Clinical Center for Gastroenterology, Southwest Hospital, Chongqing 400038, China.
World J Gastroenterol. 2004 Feb 1;10(3):419-23. doi: 10.3748/wjg.v10.i3.419.
To investigate the effects of gastric pacing on gastric emptying and plasma motilin level in a canine model of gastric motility disorders and the correlation between gastric emptying and plasma motilin level.
Ten healthy Mongrel dogs were divided into: experimental group of six dogs and control group of four dogs. A model of gastric motility disorders was established in the experimental group undergone truncal vagotomy combined with injection of glucagon. Gastric half-emptying time (GEt(1/2)) was monitored with single photon emission computerized tomography (SPECT), and the half-solid test meal was labeled with an isotope-(99m)Tc sulfur colloid. Plasma motilin concentration was measured with radioimmunoassay (RIA) kit. Surface gastric pacing at 1.1-1.2 times the intrinsic slow-wave frequency and a superimposed series of high frequency pulses (10-30 Hz) was performed for 45 min daily for a month in conscious dogs.
After surgery, GEt(1/2) in dogs undergone truncal vagotomy was increased significantly from 56.35+/-2.99 min to 79.42+/-1.91 min (P<0.001), but surface gastric pacing markedly accelerated gastric emptying and significantly decreased GEt(1/2) to 64.94+/-1.75 min (P<0.001) in animals undergone vagotomy. There was a significant increase of plasma level of motilin at the phase of IMCIII (interdigestive myoelectrical complex, IMCIII) in the dogs undergone bilateral truncal vagotomy (baseline vs vagotomy, 184.29+/-9.81 pg/ml vs 242.09+/-17.22 pg/ml; P<0.01). But plasma motilin concentration (212.55+/-11.20 pg/ml; P<0.02) was decreased significantly after a long-term treatment with gastric pacing. Before gastric pacing, GEt(1/2) and plasma motilin concentration of the dogs undergone vagotomy showed a positive correlation (r=0.867, P<0.01), but after a long-term gastric pacing, GEt(1/2) and motilin level showed a negative correlation (r=-0.733, P<0.04).
Surface gastric pacing with optimal pacing parameters can improve gastric emptying parameters and significantly accelerate gastric emptying and can resume or alter motor function in a canine model of motility disorders. Gastric emptying is correlated well with plasma motilin level before and after pacing, which suggests that motilin can modulate the mechanism of gastric pacing by altering gastric motility.
研究胃起搏对犬胃动力障碍模型胃排空及血浆胃动素水平的影响，以及胃排空与血浆胃动素水平之间的相关性。
将10只健康杂种犬分为：实验组6只犬和对照组4只犬。实验组行迷走神经切断术并注射胰高血糖素建立胃动力障碍模型。采用单光子发射计算机断层扫描（SPECT）监测胃半排空时间（GEt(1/2)），用放射性核素（99m）Tc硫胶体标记半固体试验餐。用放射免疫分析（RIA）试剂盒测定血浆胃动素浓度。对清醒犬每日进行1.1 - 1.2倍固有慢波频率的表面胃起搏及一系列叠加的高频脉冲（10 - 30Hz），持续45分钟，共1个月。
术后，行迷走神经切断术的犬GEt(1/2)从56.35±2.99分钟显著增加至79.42±1.91分钟（P<0.001），但表面胃起搏显著加速了胃排空，使行迷走神经切断术动物的GEt(1/2)显著降至64.94±1.75分钟（P<0.001）。双侧迷走神经切断术犬在消化间期肌电复合波（IMCIII）期血浆胃动素水平显著升高（基线值与迷走神经切断术后相比，184.29±9.81pg/ml对242.09±17.22pg/ml；P<0.01）。但长期胃起搏治疗后血浆胃动素浓度（212.55±11.20pg/ml；P<0.02）显著降低。胃起搏前，行迷走神经切断术犬的GEt(1/2)与血浆胃动素浓度呈正相关（r = 0.867，P<0.01），但长期胃起搏后，GEt(1/2)与胃动素水平呈负相关（r = -0.733，P<0.04）。
采用最佳起搏参数的表面胃起搏可改善胃排空参数，显著加速胃排空，并可恢复或改变动力障碍犬模型的运动功能。起搏前后胃排空与血浆胃动素水平密切相关，提示胃动素可通过改变胃动力来调节胃起搏机制。
